LOCUS       sequence-78665-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick vector for pathway engineering in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Xu P, Vansiri A, Bhan N, Koffas MA
  TITLE     ePathBrick: a synthetic biology platform for engineering metabolic 
            pathways in E. coli.
  JOURNAL   ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. 
            Epub 2012 May 4.
  PUBMED    23651248
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300016b";
            journalName: "ACS Synth Biol"; date: "2012-07-20- 20"; volume: "1"; 
            issue: "7"; pages: "256-66"
COMMENT     Sequence Label: pACM4
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    77..101
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             116..138
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             212..256
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(295..313)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      309..356
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cggccacgat gcgtccggcg tagcctagga tcgagatcga tctcgatccc gcgaaattaa
       61 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
      121 ttaactttaa gaaggagata tacatatggc agatctcaat tggatatcgg ccggccacgc
      181 gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc gctgctgcga aatttgaacg
      241 ccagcacatg gactcgtcta ctagtcgcag cttaattaac ctaaactgct gccaccgctg
      301 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctag
      361 cgaaaggagg agtcgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
      421 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
      481 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
      541 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
      601 cgccttgcgt ataatatttg cccatagtga aaacgggggc gaagaagttg tccatattgg
      661 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
      721 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
      781 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
      841 tttcagtttg ctcat
//
