LOCUS       sequence-78668-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat18
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    67..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             106..128
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             139..183
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             199..216
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
       61 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
      121 gaaggagata tacatatgaa agaaaccgct gctgctaaat tcgaacgcca gcacatggac
      181 agcccagatc tgggtaccct ggtgccacgc ggttccatgg cgatatcgga tcgggaaggt
      241 gtcaacgata acgaagaagg ctttttcagc gcccgcgaac tggttcacaa cgtggcactg
      301 cgtctggact atgtcattcc gagccgttat gatacctact ctggtcgcaa cgaagaaacg
      361 ctggaaccgg ttccgtattt tcagaaagtt ctggtcctgg tggcggcacc ggaaggtatc
      421 gcagctctgg aaaaatatta cctgaatgcg ggtgtgccga ttgaaatcaa gctgattgtg
      481 gttaccggcc tgagcggctc tggtaaagaa accctggaaa tcaagggcgg tacgccgtac
      541 ggcgcaacca cgattgctgg cggtgatggc agccgttatc agggtgaata cgtggcaggc
      601 ctggctgtta aagcgctgtt tgccaccggt aacttcgaag atgtgcgtgc agtcgtgacc
      661 ccgctgccgc gcgcactggg cgctgatgaa gttgtcaaca gccgtgcaga cca
//
