LOCUS       sequence-78678-e       15215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single component LSL vector. Contains 2x35S:Zif268:FokI outside of 
            the replicon borders. Within the replicon is the us:NPTII template. 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15215)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 15215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pZLSLD.R
FEATURES             Location/Qualifiers
     source          1..15215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1129..1323
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1559..1581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1667..1807
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1722..1741
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2668..3462)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3887..3911
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    3989..4163
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5253..5930)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    complement(6024..6044)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     terminator      complement(6089..6341)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6355..6939)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             complement(7210..7230)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(7264..7288)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(7347..7691)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             10011..10802
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10062..10081)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10672..10691
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      10829..11081
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11105..11124)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11106..11124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    12824..12848
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14148..14777
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(15211..15215,1..1063)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
       61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
      121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
      181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
      241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
      301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
      361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
      421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
      481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
      541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
      601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
      661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
      721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
      781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
      841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
      901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
      961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
     1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
     1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
     4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
     4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
     5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
     5221 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     5281 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     5341 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     5401 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     5461 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     5521 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     5581 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     5641 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
     5701 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     5761 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     5821 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     5881 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     5941 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     6001 tgtcgactat cggacgagct cggcaacttt tgtatacaaa gttgcgaatt cgccctttcc
     6061 ggaagctgtc gaggggggat caattcccga tctagtaaca tagatgacac cgcgcgcgat
     6121 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     6181 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     6241 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     6301 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggacgtcct attaaaagtt
     6361 tatctcaccg ttattaaatt tccttctcac ttcctcaagt gtcaatgtac cagctttaat
     6421 catttctcca ccaatcaaaa gctcttctac actaagaaca gctccattac aattagtgat
     6481 atgattcaat cttgtaagct gagctttgta gtttcctttg aaatgaccac tcacaaacaa
     6541 gaacttaaat tctgttactg aagatggata gactttccac cattcattag ggttgatatg
     6601 cttgtttctt gtttgattct cttcgacata tctttgcatt tcatctgctt gaccaattgg
     6661 aagattataa cctcctgaat atgccttagt atcaacgatc acaccgtaat caataggaga
     6721 tccgacagta taaattgctc cgtctggttt ccttgatcca cccaaatgtt taccacgata
     6781 accataaacc ttcataaaga actccatcac cttcatttca aggattctat cctgagttga
     6841 atttcttgcg atttcaatca attcaatata ttcatgtggc acatatttca atttatgtct
     6901 aagttcagat ttcttctctt ccaattcaga tttcactagt tccttctgac gaagatggat
     6961 ctttgtatga cgcttccttt catcacttct tgcgaactta cgaccacaga tgtcacatgc
     7021 gaatggtttc tcaccagtat gggttctgat atgtgtagtc aaatgatcgc tacgactaaa
     7081 gttcctcata cagattctac actggaaagg cttctgtcct gtgtggattc taatatgcct
     7141 agtgagttcg tctgacctac taaaacgtct atcacaagac tcaactggac aagcgtaagg
     7201 caattcaaga acctttctct ttttctttgg aggggaggaa gccatggtga agggcgaatt
     7261 cggagcctgc ttttttgtac aaacttgttg atagcttggc gcgcctcgag ggggggcccg
     7321 gtacccgggg atcctctaga gtcgaggtcc tctccaaatg aaatgaactt ccttatatag
     7381 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     7441 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     7501 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     7561 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     7621 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     7681 gaaaagtctc aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga
     7741 caagtgtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa gacgtggttg
     7801 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     7861 cggcagaggc atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc
     7921 caccttcctt ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga
     7981 ggaggtttcc ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga
     8041 ctgtatcttt gatatttttg gagtagacaa gtgtgtcgtg ctccaccatg ttgacctgca
     8101 ggcacgccaa gcttggacgc gttagcagaa ggcatgttgt tgtgactccg aggggttgcc
     8161 tcaaactcta tcttataacc ggcgtggagg catggaggca ggggtatttt ggtcatttta
     8221 atagatagtg gaaaatgacg tggaatttac ttaaagacga agtctttgcg acaagggggg
     8281 gcccacgccg aatttaatat taccggcgtg gccccccctt atcgcgagtg ctttagcacg
     8341 agcggtccag atttaaagta gaaaatttcc cgcccactag ggttaaaggt gttcacacta
     8401 taaaagcata tacgatgtga tggtatttga tggagcgtat attgtatcag gtatttccgt
     8461 tggatacgaa ttattcgtac gaccctcggc gcgccgagaa cttccagagc tttgggtacg
     8521 tcacgtggct cgagcgcgta gtcctcggta agcagtctta cttccatgat ttctttaact
     8581 atgccggaat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     8641 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     8701 tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     8761 gacaaggcac tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     8821 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     8881 ttcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     8941 cgttctactt tactggcttt ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg
     9001 ataacgtgct gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc
     9061 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     9121 tggtgattga tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     9181 cgggcaacaa gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag
     9241 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     9301 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     9361 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     9421 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     9481 gttattacgg atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     9541 aagaacttct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     9601 tggatacgtt agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     9661 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     9721 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     9781 agaaagggat cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac
     9841 gctggactgg catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa cgcagggagg
     9901 caaacaatga tatcacaact ctcctgacgc gtcatcgtcg gctacagcct cgggaattgc
     9961 tacctagctc gagcaagatc caaggagata taacaatggc ttcctcctgg attgaacaag
    10021 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    10081 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg cagggtagac
    10141 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    10201 cgcggctatc gtggctggcc acgacgggcg taccttgcgc tgctgtgctc gacgttgtca
    10261 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    10321 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    10381 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    10441 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    10501 tcgcgccagc cgaactgttc gccaggctca aggcgagaat gcccgacggc gaggatctcg
    10561 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    10621 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    10681 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    10741 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    10801 gataaccgcg gagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
    10861 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
    10921 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
    10981 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
    11041 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgtgggcccg tccagttcca
    11101 ttcgccctat agtgagtcgt attaaacctg cattaatgaa tcggccaacg cgcggggaga
    11161 ggcggtttgc gtattgagtg tacttcaagt cagtgggaaa tcaataaaat gattatttta
    11221 tgaatatatt tcattgtgca agtagataga aattacatat gttacataac acacgaaata
    11281 aacaaaaaaa gacaatccaa aaacaaacac cccaaaaaaa ataatcactt tagataaact
    11341 cgtatgagga gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa gtctccccgt
    11401 cacacatgta gtgggtgacg caattatctt taaagtaatc cttctgttga cttgtcattg
    11461 ataacatcca gtcttcgtca ggattgcaaa gaattataga agggatccca ccttttattt
    11521 tcttcttttt tccatattta gggttgacag tgaaatcaga ctggcaacct attaattgct
    11581 tccacaatgg gacgaacttg aaggggatgt cgtcgatgat attataggtg gcgtgttcat
    11641 cgtagttggt gaaatcgatg gtaccgttcc aatagttgtg tcgtccgaga cttctagccc
    11701 aggtggtctt tccggtacga gttggtccgc agatgtagag gctggggtgt cggattccat
    11761 tccttccatt gtccttgtta aatcggccat ccattcaagg tcagattgag cttgttggta
    11821 tgagacagga tgtatgtaag tataagcgtc tatgcttaca tggtatagat gggtttccct
    11881 ccaggagtgt agatcttcgt ggcagcgaag atctgattct gtgaagggcg acacatacgg
    11941 ttcaggttgt ggagggaata atttgttggc tgaatattcc agccattgaa gctttgttgc
    12001 ccattcatga gggaattctt ccttgatcat gtcaagatat tcctccttag acgttgcagt
    12061 ctggataata gttctccatc gtgcgtcaga tttgcgagga gaaaccttat gatctcggaa
    12121 atctcctctg gttttaatat ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt
    12181 tctagctggc tggatattag ggtgatttcc ttcaaaatcg aaaaaagaag gatccctaat
    12241 acaaggtttt ttatcaagct ggagaagagc atgatagtgg gtagtgccat cttgatgaag
    12301 ctcagaagca acaccaagga agaaaataag aaaaggtgtg agtttctccc agagaaactg
    12361 gaataaatca tctctttgag atgagcactt gggataggta aggaaaacat atttagattg
    12421 gagtctgaag ttcttactag cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa
    12481 ctctatctta taaccggcgt ggaggcatgg aggcaggggt attttggtca ttttaataga
    12541 tagtggaaaa tgacgtggaa tttacttaaa gacgaagtct ttgcgacaag ggggggccca
    12601 cgccgaattt aatattaccg gcgtggcccc cccttatcgc gagtgcttta gcacgagcgg
    12661 tccagattta aagtagaaaa tttcccgccc actagggtta aaggtgttca cactataaaa
    12721 gcatatacga tgtgatggta tttgatggag cgtatattgt atcaggtatt tccgttggat
    12781 acgaattatt cgtacgaccc tcatagttta aactatcagt gtttgacagg atatattggc
    12841 gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa
    12901 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
    12961 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
    13021 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
    13081 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
    13141 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
    13201 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
    13261 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
    13321 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
    13381 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
    13441 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
    13501 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
    13561 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
    13621 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
    13681 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
    13741 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
    13801 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
    13861 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
    13921 gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc
    13981 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
    14041 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
    14101 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
    14161 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
    14221 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
    14281 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
    14341 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
    14401 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
    14461 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
    14521 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
    14581 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
    14641 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
    14701 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
    14761 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
    14821 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
    14881 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
    14941 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
    15001 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
    15061 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
    15121 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
    15181 gaacccccaa gcccgaggaa tcggcgtgac ggtcg
//
