LOCUS       sequence-78686-e       19573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19573)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 19573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: p35ST
FEATURES             Location/Qualifiers
     source          1..19573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..27)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11..27)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(24..46)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    35..51
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(59..65,66..83,84..89))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    104..125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        316..993
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1061..2086
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2126..2300
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2378..2402
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2827..3621
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3708..4296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4197..4216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4450..4467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4482..4622
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4548..4567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4708..4730
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4966..5160
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5226..6293)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6727..7356)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8868..8890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8882..8899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9326..9670
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    9729..9753
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9775..9840
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9847..9867
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12895..12948
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             12955..13020
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             13027..13047
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15676..16263
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      16277..16529
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    16574..16594
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             18071..18862
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(18122..18141)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     18732..18751
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      18889..19141
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(19165..19184)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(19166..19184)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(19231..19255)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
       61 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      121 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      181 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc
      241 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      301 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      361 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      421 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      481 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      541 gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacactc tcgtctactc
      601 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
      661 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
      721 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
      781 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
      841 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
      901 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccttc ctctatataa
      961 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
     1021 tctctctcga gctttcgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac
     1081 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     1141 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     1201 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     1261 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta gcgagagcct
     1321 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     1381 actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg cggccgatct
     1441 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     1501 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     1561 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     1621 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     1681 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     1741 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     1801 ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg cgtatatgct
     1861 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     1921 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     1981 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     2041 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta gatgccgacc
     2101 ggatctgtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     2161 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     2221 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     2281 ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg
     2341 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
     2401 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
     2461 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
     2521 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
     2581 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
     2641 ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt
     2701 tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt
     2761 cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa
     2821 taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc
     2881 tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat
     2941 gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa
     3001 cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac
     3061 tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc
     3121 tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc
     3181 atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc
     3241 cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac
     3301 tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa
     3361 aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg
     3421 aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg
     3481 tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc
     3541 gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt
     3601 ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt
     3661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4321 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4381 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4441 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     4501 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     4561 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     4621 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4681 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4741 catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac
     4801 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
     4861 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
     4921 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
     4981 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
     5041 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
     5101 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
     5161 ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
     5221 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
     5281 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
     5341 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
     5401 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
     5461 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
     5521 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
     5581 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
     5641 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
     5701 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
     5761 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
     5821 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
     5881 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
     5941 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
     6001 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
     6061 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc
     6121 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
     6181 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
     6241 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc
     6301 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
     6361 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
     6421 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
     6481 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
     6541 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
     6601 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
     6661 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
     6721 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
     6781 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
     6841 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
     6901 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
     6961 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
     7021 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
     7081 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
     7141 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
     7201 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
     7261 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
     7321 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
     7381 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
     7441 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
     7501 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
     7561 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
     7621 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
     7681 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
     7741 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
     7801 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
     7861 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
     7921 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
     7981 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
     8041 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
     8101 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
     8161 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
     8221 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
     8281 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
     8341 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
     8401 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
     8461 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
     8521 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
     8581 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttgg cgtgcctgca ggtcaacatg gtggagcacg
     8941 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     9001 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     9061 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     9121 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     9181 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     9241 attgatgtga taacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     9301 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     9361 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     9421 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     9481 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     9541 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
     9601 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     9661 tggagaggac ctcgactcta gaggatcccc gggtaccggg ccccccctcg aggcgcgcca
     9721 agctatcaac aagtttgtac aaaaaagcag gctccgaatt cgcccttcac catggattat
     9781 aaggatcacg atggcgacta caaggaccac gatattgact acaaagacga tgacgataaa
     9841 atggctccta agaaaaagcg caaagtcggt atccatggcg ttccctctag aatggtggat
     9901 ctacgcacgc tcggctacag tcagcagcag caagagaaga tcaaaccgaa ggtgcgttcg
     9961 acagtggcgc agcaccacga ggcactggtg ggccatgggt ttacacacgc gcacatcgtt
    10021 gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcacgtatca gcacataatc
    10081 acggcgttgc cagaggcgac acacgaagac atcgttggcg tcggcaaaca gtggtccggc
    10141 gcacgcgccc tggaggcctt gctcacggat gcgggggagt tgagaggtcc gccgttacag
    10201 ttggacacag gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc aatggaggca
    10261 gtgcatgcat cgcgcaatgc actgacgggt gcccccctga acctgacccc ggaccaagtg
    10321 gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg
    10381 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
    10441 aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
    10501 catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg caagcaagcg
    10561 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
    10621 caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg
    10681 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
    10741 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
    10801 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag
    10861 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
    10921 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
    10981 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
    11041 atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
    11101 ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc
    11161 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
    11221 ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa
    11281 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
    11341 gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg
    11401 ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat cgccagccac
    11461 gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
    11521 catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg
    11581 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
    11641 caagtggtgg ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg
    11701 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
    11761 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
    11821 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag
    11881 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
    11941 ccggaccaag tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg
    12001 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
    12061 atcgccagca acggtggcgg caagcaagcg ctcgaaagca ttgtggccca gctgagccgg
    12121 cctgatccgg cgttggccgc gttgaccaac gaccacctcg tcgccttggc ctgcctcggc
    12181 ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga
    12241 agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg ttgccggatc ccagctggtg
    12301 aagtccgagc tggaagaaaa aaagagcgag ctgcgccaca agctcaagta cgtgccccac
    12361 gagtacatcg agctgatcga gatcgcccgc aacagcaccc aagaccgcat cctggagatg
    12421 aaagtgatgg agttcttcat gaaggtgtac ggctaccgcg gcaagcacct gggcggctcc
    12481 cgcaagcccg atggcgccat ctacaccgtg ggctccccca tcgactatgg cgtcattgtc
    12541 gacaccaagg cctactccgg cggctacaac ttacccatcg gtcaggccga cgagatgcaa
    12601 cgctacgtga aggagaacca gacccgcaat aagcacatta atcccaacga gtggtggaag
    12661 gtgtacccct cctccgtgac cgagttcaaa ttcctgttcg tgtccggcca cttcaagggc
    12721 aattataagg cccaactgac ccgcctgaac cacaagacca actgcaacgg cgccgtgctg
    12781 tccgtggagg aactgctgat cggcggcgag atgatcaagg ctggtaccct gaccctggaa
    12841 gaggtgcgcc gcaagttcaa caatggtgaa atcaatttca ggtccggcgg cggagagggc
    12901 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag gatggactac
    12961 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
    13021 atggccccca agaagaagag gaaggtgggc attcacgggg tgccggctag aatggtggat
    13081 ctacgcacgc tcggctacag tcagcagcag caagagaaga tcaaaccgaa ggtgcgttcg
    13141 acagtggcgc agcaccacga ggcactggtg ggccatgggt ttacacacgc gcacatcgtt
    13201 gcgctcagcc aacacccggc agcgttaggg accgtcgctg tcacgtatca gcacataatc
    13261 acggcgttgc cagaggcgac acacgaagac atcgttggcg tcggcaaaca gtggtccggc
    13321 gcacgcgccc tggaggcctt gctcacggat gcgggggagt tgagaggtcc gccgttacag
    13381 ttggacacag gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc aatggaggca
    13441 gtgcatgcat cgcgcaatgc actgacgggt gcccccctga acctgacccc ggaccaagtg
    13501 gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg
    13561 ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac
    13621 aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
    13681 catggcctga ccccggacca agtggtggct atcgccagca acaatggcgg caagcaagcg
    13741 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac
    13801 caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg
    13861 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc
    13921 agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
    13981 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag
    14041 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
    14101 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
    14161 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
    14221 atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
    14281 ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc
    14341 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
    14401 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
    14461 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
    14521 gtggctatcg ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg
    14581 ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat cgccagccac
    14641 gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac
    14701 catggcctga ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg
    14761 ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac
    14821 caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg
    14881 ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc
    14941 agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc
    15001 caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag
    15061 caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc
    15121 ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg
    15181 cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct
    15241 atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg
    15301 ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag caacattggc
    15361 ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc
    15421 ctgaccccgg accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa
    15481 agcattgtgg cccagctgag ccggcctgat ccggcgttgg ccgcgttgac caacgaccac
    15541 ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa aaagggattg
    15601 ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg cacgtcccat
    15661 cgcgttgccg gatctcaact agtcaaaagt gaactggagg agaagaaatc tgaacttcgt
    15721 cataaattga aatatgtgcc tcatgaatat attgaattaa ttgaaattgc cagaaattcc
    15781 actcaggata gaattcttga aatgaaggta atggaatttt ttatgaaagt ttatggatat
    15841 agaggtaaac atttgggtgg atcaaggaaa ccggacggag caatttatac tgtcggatct
    15901 cctattgatt acggtgtgat cgtggatact aaagcttata gcggaggtta taatctgcca
    15961 attggccaag cagatgaaat ggagcgatat gtcgaagaaa atcaaacacg aaacaaacat
    16021 ctcaacccta atgaatggtg gaaagtctat ccatcttctg taacggaatt taagttttta
    16081 tttgtgagtg gtcactttaa aggaaactac aaagctcagc ttacacgatt aaatcatatc
    16141 actaattgta atggagctgt tcttagtgta gaagagcttt taattggtgg agaaatgatt
    16201 aaagccggca cattaacctt agaggaagtg agacggaaat ttaataacgg cgagataaac
    16261 ttttaatagg acgtccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
    16321 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
    16381 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
    16441 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
    16501 gcgcgcggtg tcatctatgt tactagatcg ggaattgatc ccccctcgac agcttccgga
    16561 aagggcgaat tcgcaacttt gtatacaaaa gttgccgagc tcgcggccgc agcagtctta
    16621 cttccatgat ttctttaact atgccggaat ccatcgcagc gtaatgctct acaccacgcc
    16681 gaacacctgg gtggacgata tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc
    16741 gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc gttgaactgc gtgatgcgga
    16801 tcaacaggtg gttgcaactg gacaaggcac tagcgggact ttgcaagtgg tgaatccgca
    16861 cctctggcaa ccgggtgaag gttatctcta tgaactgtgc gtcacagcca aaagccagac
    16921 agagtgtgat atctacccgc ttcgcgtcgg catccggtca gtggcagtga agggcgaaca
    16981 gttcctgatt aaccacaaac cgttctactt tactggcttt ggtcgtcatg aagatgcgga
    17041 cttgcgtggc aaaggattcg ataacgtgct gatggtgcac gaccacgcat taatggactg
    17101 gattggggcc aactcctacc gtacctcgca ttacccttac gctgaagaga tgctcgactg
    17161 ggcagatgaa catggcatcg tggtgattga tgaaactgct gctgtcggct ttaacctctc
    17221 tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg aagaggcagt
    17281 caacggggaa actcagcaag cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa
    17341 aaaccaccca agcgtggtga tgtggagtat tgccaacgaa ccggataccc gtccgcaagg
    17401 tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc
    17461 gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc gataccatca gcgatctctt
    17521 tgatgtgctg tgcctgaacc gttattacgg atggtatgtc caaagcggcg atttggaaac
    17581 ggcagagaag gtactggaaa aagaacttct ggcctggcag gagaaactgc atcagccgat
    17641 tatcatcacc gaatacggcg tggatacgtt agccgggctg cactcaatgt acaccgacat
    17701 gtggagtgaa gagtatcagt gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt
    17761 cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat tttgcgacct cgcaaggcat
    17821 attgcgcgtt ggcggtaaca agaaagggat cttcactcgc gaccgcaaac cgaagtcggc
    17881 ggcttttctg ctgcaaaaac gctggactgg catgaacttc ggtgaaaaac cgcagcaggg
    17941 aggcaaacaa cgcagggagg caaacaatga tatcacaact ctcctgacgc gtcatcgtcg
    18001 gctacagcct cgggaattgc tacctagctc gagcaagatc caaggagata taacaatggc
    18061 ttcctcctgg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
    18121 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
    18181 gctgtcagcg cagggtagac cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
    18241 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg taccttgcgc
    18301 tgctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
    18361 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
    18421 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
    18481 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
    18541 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagaat
    18601 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
    18661 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    18721 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    18781 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    18841 ccttcttgac gagttcttct gataaccgcg gagagctcga atttccccga tcgttcaaac
    18901 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
    18961 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
    19021 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
    19081 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
    19141 cgtgggcccg tccagttcca ttcgccctat agtgagtcgt attaaacctg cattaatgaa
    19201 tcggccaacg cgcggggctg caggtcgacg acccagcttt cttgtacaaa gtggttcgat
    19261 aattccttaa ttaactagtt ctagagcggc cgcccaccgc ggtggagctc gaatttcccc
    19321 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
    19381 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
    19441 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
    19501 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
    19561 atgttactga att
//
