LOCUS       sequence-78696-e       16691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLSL with GUS coding sequence followed by 3 kb of filler sequence 
            Rep is not included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16691)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 16691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGUS
FEATURES             Location/Qualifiers
     source          1..16691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    529..553
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             575..640
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             647..667
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             692..2503
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      2511..2763
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2808..2828
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3859..4650
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3910..3929)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4520..4539
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4677..4929
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(5942..5966)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6732..7076
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(7788..7804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7788..7804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7801..7823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7812..7828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7836..7842,7843..7860,7861..7866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7881..7902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8093..8770
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             8838..9863
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    9904..10078
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10156..10180
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10605..11399
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11486..12074
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11975..11994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12228..12245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12260..12400
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12326..12345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12486..12508
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12744..12938
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13004..14071)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(14505..15134)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    16434..16458
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     16646..16668
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16660..16677
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16661..16677
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggta gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt
       61 ataaccggcg tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa
      121 atgacgtgga atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt
      181 taatattacc ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt
      241 aaagtagaaa atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg
      301 atgtgatggt atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat
      361 tcgtacgacc ctccctaaga ttcttgattg tttataaaac caaatctcat tgtctttgtt
      421 gtgtattgtt tgcaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
      481 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttctac aagtttgtac
      541 aaaaaagcag gctccgaatt cgcccttcac catggattat aaggatcacg atggcgacta
      601 caaggaccac gatattgact acaaagacga tgacgataaa atggctccta agaaaaagcg
      661 caaagtcggt atccatggcg ttccctctag aatgttacgt cctgtagaaa ccccaacccg
      721 tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa actgtggaat
      781 tgatcagcgt tggtgggaaa gcgcgttaca agaaagccgg gcaattgctg tgccaggcag
      841 ttttaacgat cagttcgccg atgcagatat tcgtaattat gcgggcaacg tctggtatca
      901 gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc
      961 ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg
     1021 ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat
     1081 caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa tggtgattac
     1141 cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact atgccggaat
     1201 ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata tcaccgtggt
     1261 gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg
     1321 tgatgtcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac
     1381 tagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag gttatctcta
     1441 tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc ttcgcgtcgg
     1501 catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac cgttctactt
     1561 tactggcttt ggtcgtcatg aagatgcgga cttgcgtggc aaaggattcg ataacgtgct
     1621 gatggtgcac gaccacgcat taatggactg gattggggcc aactcctacc gtacctcgca
     1681 ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg tggtgattga
     1741 tgaaactgct gctgtcggct ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa
     1801 gccgaaagaa ctgtacagcg aagaggcagt caacggggaa actcagcaag cgcacttaca
     1861 ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat
     1921 tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga
     1981 agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga
     2041 cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc gttattacgg
     2101 atggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa aagaacttct
     2161 ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg tggatacgtt
     2221 agccgggctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt gtgcatggct
     2281 ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa
     2341 tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat
     2401 cttcactcgc gaccgcaaac cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg
     2461 catgaacttc ggtgaaaaac cgcagcaggg aggcaaacaa tgagacgtcc gatcgttcaa
     2521 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     2581 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     2641 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     2701 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     2761 atcgggaatt gatcccccct cgacagcttc cggaaagggc gaattcgcaa ctttgtatac
     2821 aaaagttgcc gagctcgcgg ccgcagtgcg agatcgggcc ggcctggccc tgttttgatt
     2881 gatgtaccta aggatattca gcaacaattg gtgatacctg actgggatca gccaatgagg
     2941 ttgcctggtt acatgtctag gttacctaaa ttgcccaatg agatgctttt agaacaaatt
     3001 gttaggctta tttctgagtc aaagaagcct gttttgtatg tggggggtgg gtgttcgcaa
     3061 tcgagtgagg agttgagacg attcgtggag ctcaccggta tccccgtggc aagtactttg
     3121 atgggtcttg gagcttttcc aactggggat gagctttccc tttcaatgtt gggtatgcat
     3181 ggtactgttt atgctaatta tgctgtggac agtagtgatt tattgctcgc atttggggtg
     3241 aggtttgatg atagagttac tggaaagtta gaagcttttg ctagccgagc gaaaattgtt
     3301 cacattgata ttgattcagc tgagattgga aagaacaagc agcctcatgt ttccatttgt
     3361 gcggatatca agttggcgtt acagggtttg aattcgatat tggagagtaa ggaaggtaaa
     3421 ctgaagttgg atttttctgc ttggaggcag gagttgacgg tgcagaaagt gaagtacccg
     3481 ttgaatttta aaacttttgg tgatgctatt cctccgcaat atgctatcca ggttctagat
     3541 gagttaacta atgggagtgc tattataagt accggtgttg ggcagcacca gatgtgggct
     3601 gctcaatatt ataagtacag aaagccacgc caatggttga catctggtgg attaggagcg
     3661 atgggatttg gtttgcccgc tgctattggt gcggctgttg gaagacctga tgaagttgtg
     3721 gttgacattg atggtgatgg cagtttcatc atgaatgtgc aggagctagc aactattaag
     3781 gtggagaatc tcccagttaa gattatgtta ctgaataatc aacacttggg aatggttgta
     3841 caaatggctt cctcctggat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3901 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3961 gtgttccggc tgtcagcgca gggtagaccg gttctttttg tcaagaccga cctgtccggt
     4021 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgta
     4081 ccttgcgctg ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4141 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4201 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4261 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4321 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4381 gcgagaatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     4441 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     4501 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     4561 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     4621 ttctatcgcc ttcttgacga gttcttctga taaccgcgga gagctcgaat ttccccgatc
     4681 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     4741 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     4801 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     4861 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     4921 tactagatcg tgggcctcga ggatcgattc tataaggcta acagagcaca cacatacctg
     4981 gggaatcctt ctaatgaggc ggagatcttt cctaatatgt tgaaatttgc agaggcttgt
     5041 ggcgtacctg ctgcgagagt gacacacagg gatgatctta gagcggctat tcaaaagatg
     5101 ttagacactc ctgggccata cttgttggat gtgattgtac ctcatcagga acatgttcta
     5161 cctatgattc ctaccggagg ggctttcaaa gacgtcatca cagagggtga cgggagaagt
     5221 tcctattgac tttgaggtgc tacagagcta gttctaggcc ttgtattatc taaaataaac
     5281 ttctattaaa ccaaaaatgt tatgtctatt agtttgttat tagtttttcc gtggctttgc
     5341 tcattgtcag tgttgtacta ttaagtagtt gatatttatg tttgctttaa gttttgcatc
     5401 atctcgcttt ggttttgaat gtgaaggatt tcagcaatgt ttcattctct attcgcaaca
     5461 tccagtcggt atccggagct ctatgtagta tgtctggaga ttaatttcta gtggagtagt
     5521 ttagtgcgat aaagttagct tgttccacat ttttatttcg taacctgggt cagattggaa
     5581 cttcctcttt aggttggatg caatccctat ttgggctttc tcttaatttc attattgaaa
     5641 ttgttggctt ttaatctgag caagttgatt tgcagctttc tctcttgagt cctagcgagc
     5701 aatacgttat ctctgtctcc tatttcttag tggataatct tatgatggaa atctgtggag
     5761 ataggaaact gttgactgct cattctctct ttgtgaggcg tctgacagtt acgctttcaa
     5821 tctatagcag ttcgatcaga ctttctttgc ttataacaat actagataca gttggaccgt
     5881 gtagcacggg cccaacaatt tgtgtaacgt tatctttaat tcaaaatatc gcaggtcgac
     5941 gacccagctt tcttgtacaa agtgggagtg tacttcaagt cagtgggaaa tcaataaaat
     6001 gattatttta tgaatatatt tcattgtgca agtagataga aattacatat gttacataac
     6061 acacgaaata aacaaaaaaa gacaatccaa aaacaaacac cccaaaaaaa ataatcactt
     6121 tagataaact cgtatgagga gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa
     6181 gtctccccgt cacacatgta gtgggtgacg caattatctt taaagtaatc cttctgttga
     6241 cttgtcattg ataacatcca gtcttcgtca ggattgcaaa gaattataga agggatccca
     6301 ctcgagccaa gcttggcgtg tctgcaggtc aacatggtgg agcacgacac acttgtctac
     6361 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
     6421 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
     6481 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     6541 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     6601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
     6661 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac
     6721 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
     6781 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
     6841 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
     6901 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     6961 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     7021 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacctcg
     7081 actctagagg atcccccttc ctctatataa ggaagttcat ttcatttgga gaggtaagtt
     7141 tcacttcaca cattattact gtcttctaat acaaggtttt ttatcaagct ggagaagagc
     7201 atgatagtgg gtagtgccat cttgatgaag ctcagaagca acaccaagga agaaaataag
     7261 aaaaggtgtg agtttctccc agagaaactg gaataaatca tctctttgag atgagcactt
     7321 gggataggta aggaaaacat atttagattg gagtctgaag ttcttactag cagaaggcat
     7381 gttgttgtga ctccgagggg ttgcctcaaa ctctatctta taaccggcgt ggaggcatgg
     7441 aggcaggggt attttggtca ttttaataga tagtggaaaa tgacgtggaa tttacttaaa
     7501 gacgaagtct ttgcgacaag ggggggccca cgccgaattt aatattaccg gcgtggcccc
     7561 cccttatcgc gagtgcttta gcacgagcgg tccagattta aagtagaaaa tttcccgccc
     7621 actagggtta aaggtgttca cactataaaa gcatatacga tgtgatggta tttgatggag
     7681 cgtatattgt atcaggtatt tccgttggat acgaattatt cgtacgaccc tccctgcagg
     7741 tcgactctag aggatccccg ggtaccgagc tcgaattcgt aatcatggtc atagctgttt
     7801 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7861 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7921 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7981 gggagaggcg gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc
     8041 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
     8101 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
     8161 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
     8221 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     8281 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     8341 gtgataacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     8401 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     8461 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
     8521 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
     8581 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     8641 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     8701 aatcccacta tccttcgcaa gaccttcctc tatataagga agttcatttc atttggagag
     8761 gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc
     8821 cgggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     8881 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     8941 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     9001 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     9061 cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtg
     9121 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg
     9181 tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     9241 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     9301 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     9361 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     9421 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     9481 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     9541 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     9601 agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct
     9661 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     9721 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     9781 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     9841 ctcgtccgag ggcaaagaaa tagagtagat gccgacgcgg atctgtcgat cgacaagctc
     9901 gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg ttcctatagg
     9961 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
    10021 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatccc
    10081 ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
    10141 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
    10201 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
    10261 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
    10321 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
    10381 tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc
    10441 gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat
    10501 ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct
    10561 tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa
    10621 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
    10681 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
    10741 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    10801 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    10861 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    10921 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
    10981 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
    11041 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta
    11101 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
    11161 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    11221 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    11281 ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    11341 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt
    11401 acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
    11641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12181 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12241 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    12301 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    12361 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
    12421 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
    12481 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
    12541 gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg
    12601 gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac
    12661 gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt
    12721 cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa
    12781 gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca
    12841 tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa
    12901 tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt
    12961 tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg
    13021 cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct
    13081 cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac
    13141 agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc
    13201 tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg
    13261 gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga
    13321 tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc
    13381 tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc
    13441 cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg
    13501 tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt
    13561 ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt
    13621 tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg
    13681 ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca
    13741 cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc
    13801 gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc
    13861 ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc
    13921 ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt
    13981 gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc
    14041 cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg cttgggggtt
    14101 ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt
    14161 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    14221 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    14281 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    14341 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    14401 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    14461 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    14521 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    14581 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    14641 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    14701 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    14761 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    14821 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    14881 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    14941 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    15001 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    15061 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    15121 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    15181 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    15241 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    15301 cggccaggat ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc
    15361 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    15421 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    15481 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    15541 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    15601 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    15661 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    15721 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    15781 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    15841 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    15901 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    15961 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    16021 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    16081 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    16141 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    16201 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    16261 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    16321 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    16381 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
    16441 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
    16501 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
    16561 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
    16621 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
    16681 aagcttgcat g
//
