LOCUS       sequence-78710-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of the human TPI1 Ile170Val allele in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Gruning NM, Du D, Keller MA, Luisi BF, Ralser M
  TITLE     Inhibition of triosephosphate isomerase by phosphoenolpyruvate in 
            the feedback-regulation of glycolysis.
  JOURNAL   Open Biol. 2014 Mar 5;4(3):130232. doi: 10.1098/rsob.130232.
  PUBMED    24598263
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2014-03-5"; volume: "4(3):130232. doi"; pages: " 
            10.1098/rsob.130232"
COMMENT     Sequence Label: pET20b- hTPI Ile170Val
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..44)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgggctttgt tagcagccgg atctcagtgg tggtggtggt ggtgctcgag ttgtttggca
       61 ttgatgatgt ccacgaattc gggcttgagg gaagcaccac ccacaaggaa gccatccaca
      121 tcaggctggc tggccagctc cttgcaggtt gccccagtca cagagcctcc ataaatgata
      181 cgggtgctct gagccaccgc atcagagacg ttggacttca gccatcctcg gagcttctcg
      241 tgtacttcct gggcctgttg gggtgttgca gtcttgccag taccaacggc ccacacaggc
      301 tcataggcca ggacgacctt gctccagtcc ttcacgttat ctgcgatgac ctttgtctgc
      361 tcgaaaacaa ccttctcagt gatgccagct tccctttcat ctagcttctc cccaatgcag
      421 gcgattactc cgagtccctc tgccagagca tgggccactt tctgcccaat cagctcatct
      481 gactccccaa agacatgcct tctctctgag tgccccagga ccacccacgt ggctccgcag
      541 tctttgatca tgccagggct gatctcccca gtaaaagccc cattagtcac tttgtagcag
      601 ttctgcgcag ccacagcaat cttgggatct agcttctgcc gggcgaagtc gatataggca
      661 gtagggggag cacaaaccac ctcggtgtcg gccggcacct tggccgcgtt cagagtgccg
      721 atgagctccc ccagactctg cttccgcccg ttcatcttcc agtttccccc aacga
//
