LOCUS       sequence-78730-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for N-terminal his tagged S.aureus SrtA delta 59.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, 
            Ploegh HL
  TITLE     Site-specific C-terminal and internal loop labeling of proteins 
            using sortase-mediated reactions.
  JOURNAL   Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub
            2013 Aug 29.
  PUBMED    23989673
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.101"; journalName: "Nat Protoc"; date: 
            "2013-09"; volume: "8"; issue: "9"; pages: "1787-99"
COMMENT     Sequence Label: pET28a-SrtAdelta59
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             588..605
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(658..676)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      672..719
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(843..862)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgagaggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatgcaa gctaaacctc aaattccgaa
      121 agataaatcg aaagtggcag gctatattga aattccagat gctgatatta aagaaccagt
      181 atatccagga ccagcaacac ctgaacaatt aaatagaggt gtaagctttg cagaagaaaa
      241 tgaatcacta gatgatcaaa atatttcaat tgcaggacac actttcattg accgtccgaa
      301 ctatcaattt acaaatctta aagcagccaa aaaaggtagt atggtgtact ttaaagttgg
      361 taatgaaaca cgtaagtata aaatgacaag tataagagat gttaagccta cagatgtagg
      421 agttctagat gaacaaaaag gtaaagataa acaattaaca ttaattactt gtgatgatta
      481 caatgaaaag acaggcgttt gggaaaaacg taaaatcttt gtagctacag aagtcaaata
      541 aggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc
      601 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      661 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      721 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
      781 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      841 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      901 tctaaatcgg gggctccctt t
//
