LOCUS       sequence-78731-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for N-terminal his tagged S.pyogenes SrtA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, 
            Ploegh HL
  TITLE     Site-specific C-terminal and internal loop labeling of proteins 
            using sortase-mediated reactions.
  JOURNAL   Nat Protoc. 2013 Sep;8(9):1787-99. doi: 10.1038/nprot.2013.101. Epub
            2013 Aug 29.
  PUBMED    23989673
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.101"; journalName: "Nat Protoc"; date: 
            "2013-09"; volume: "8"; issue: "9"; pages: "1787-99"
COMMENT     Sequence Label: pET28a-SpySrtA
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             463..480
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(533..551)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      547..594
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(718..737)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gccataccag agcttggcat taatttacca atttttaaag gtttaggaaa tactgagctt
       61 atttatggcg caggaacgat gaaagaagaa caagttatgg gaggagaaaa taattattct
      121 cttgccagtc atcatatttt tggaattaca ggttcatctc aaatgctctt ttcgccgctt
      181 gaaagagcac aaaatgggat gtccatctat ttaacagata aagaaaaaat ttacgaatac
      241 atcataaaag atgttttcac ggtagctcct gaacgcgttg atgttatcga tgatacagct
      301 ggtctcaaag aagtgacttt agtgacttgt acagatatcg aagcaacaga acgtattatt
      361 gtcaaaggag aactaaaaac agaatacgac tttgataaag cgcccgccga tgtattgaaa
      421 gcttttaatc attcttataa ccaagtatct acctagctcg agcaccacca ccaccaccac
      481 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
      541 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
      601 ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      661 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      721 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      781 atcgggggct ccctttaggg ttccgattta
//
