LOCUS       sequence-78739-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid for C-terminal his tagged, calcium independent 
            S.aureus SrtA with enhanced catalytic activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  TITLE     unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pet30b-7M SrtA
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             481..498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(551..569)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      565..612
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(736..755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagaatacat atgcaagcta aacctcaaat tccgaaagat
       61 aaatcaaaag tggcaggcta tattgaaatt ccagatgctg atattaaaga accagtatat
      121 ccaggaccag caacacgcga acaattaaat agaggtgtaa gctttgcaaa agaaaatcaa
      181 tcactagatg atcaaaatat ttcaattgca ggacacactt tcattgaccg tccgaactat
      241 caatttacaa atcttaaagc agccaaaaaa ggtagtatgg tgtactttaa agttggtaat
      301 gaaacacgta agtataaaat gacaagtata agaaacgtta agccaacagc tgtagaagtt
      361 ctagatgaac aaaaaggtaa agataaacaa ttaacattaa ttacttgtga tgattacaat
      421 gaagagacag gcgtttggga aacacgtaaa atctttgtag ctacagaagt caaactcgag
      481 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      541 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      601 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      781 ttccccgtca agctctaaat cggggggctc cctttagggt tccgatttag tgctttacgg
      841 cacctcgacc ccaaaaaact tgatta
//
