LOCUS       sequence-78754-e         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Segarra M, Vilardell C, Matsumoto K, Esparza J, Lozano E, 
            Serra-Pages C, Urbano-Marquez A, Yamada KM, Cid MC
  TITLE     Dual function of focal adhesion kinase in regulating 
            integrin-induced MMP-2 and MMP-9 release by human T lymphoid cells.
  JOURNAL   FASEB J. 2005 Nov;19(13):1875-7.
  PUBMED    16260653
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J."; 
            date: "2005-11"; volume: "19(13)"; pages: "1875-7"
COMMENT     Sequence Label: pRKVSV FAT
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        226..244
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     226..243
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     257..276
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      322..331
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             334..366
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             367..399
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 gtgaaccgtc agaagcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
       61 ccgggaccga tccagcctcc gcggccggga acggtgcatt ggaacgcgga ttccccgtgc
      121 caagagtgac gtaagtaccg cctatagagt ctataggccc acccccttgg cttcgttaga
      181 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
      241 tagaataaca tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcac
      301 ctcggttcta tcgattcagc cgccaccatg ggatacaccg acatcgagat gaaccggctg
      361 ggcaagtaca ccgacatcga gatgaaccgg ctgggcaagg gatcccgagg cagcatcgac
      421 agggaagacg ggagttttca gggtccgact ggaaaccaac acatctatca gcctgtgggg
      481 aagccagatc ctgcagctcc accaaagaaa cctcctcgcc ctggagcacc tggccaccta
      541 agcaacctgt ccagcatcag cagccctgca gacagctaca atgagggtgt caagcttcag
      601 ccccaggaaa tcagcccccc tcccactgcc aaccttgacc ggtccaatga caaggtatat
      661 gaaaatgtga caggcctagt gaaggctgtc atcgagatgt ccagcaaaat ccagccagct
      721 cctccagaag agtacgtccc tatggtgaag gaagttggcc tggccctgcg gaccttacta
      781 gccacggtgg atgagaccat tcctgctctt ccagccagca ctcatcgaga gatcgagatg
      841 gcacagaagc tgctgaactc cgacttaggc gagctcatca gcaagatgaa gctggcgcag
      901 cagtacgtca tgaccagcct gcagc
//
