LOCUS       sequence-78757-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 C723S C724V M727C mutant with an N-terminal 
            3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing 
            convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 C723S C724V M727C (NT...
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccatgtgtac gtgtccacag atcatcaaac caacattttt tgtgaaatcg tgaacaagat
       61 gaagtaaggc cggccgtgag tttggcgctc ctgtcataac aagacactgt ggcctaaagt
      121 ttttcacatg gtcctccacg ccagaaagcc taatactatg ttgcagggca ttgaggtaag
      181 tcagggcttg tgtagaggat ccccaattca catcgggttt tttgtaggta acataaatat
      241 agagaccaag gactatcacg taggttagca aagcagccca ccagttaatg acgaaacata
      301 ctatcacaga aagaattgct ccaagaagtg agatccacat attgtagtat ttaaatgccg
      361 gtcgccatcc tgggcttttt gccagtgaag catggaatac tgaaaaattg atgagggcat
      421 agctggcaag gaagaagttt gagataattg gtgcaataac attcagttcg gcaattaaga
      481 tgaatccaag tgcaattaag aatgttaaga tgtagccacg taatggctca ttatttttcc
      541 cataaccttt agcaaacatc tggaaagcgg ggtagatgtt gtctttacat agggcctgaa
      601 atatcttggg agcactcact agggatgcta atgcgctaga aagagtggca gagaatatgc
      661 ccgcggaaat tagtggtgta aatcctgaca ccatactcat tacctgaaag ttgttcatta
      721 ggccgtagct acaaggactg gattcacaag atgaaaaatc aaagtttaat ttgcaggctg
      781 cgctagtaca gtttgttagc tctgttacga tagtgtcatt aacattacca gtg
//
