LOCUS       sequence-78763-e        4320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express DsRed monomer-tagged hAIM2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
  TITLE     AIM2 activates the inflammasome and cell death in response to 
            cytoplasmic DNA.
  JOURNAL   Nature. 2009 Mar 26;458(7237):509-13. doi: 10.1038/nature07710. Epub
            2009 Jan 21.
  PUBMED    19158676
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07710"; journalName: "Nature"; date: "2009-03-26- 
            26"; volume: "458"; issue: "7237"; pages: "509-13"
COMMENT     Sequence Label: pDsRed-monomer-hAIM2
FEATURES             Location/Qualifiers
     source          1..4320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      1290..1299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1296..1973
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     1694..1713
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2110..2132)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2232..2251
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2329..2347)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2415..2519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2520..2588,2589..3380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2738..2757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3551..4139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4040..4059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4293..4310
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta
      241 tggagagtaa atacaaggag atactcttgc taacaggcct ggataacatc actgatgagg
      301 aactggatag gtttaagttc tttctttcag acgagtttaa tattgccaca ggcaaactac
      361 atactgcaaa cagaatacaa gtagctacct tgatgattca aaatgctggg gcggtgtctg
      421 cagtgatgaa gaccattcgt atttttcaga agttgaatta tatgcttttg gcaaaacgtc
      481 ttcaggagga gaaggagaaa gttgataagc aatacaaatc ggtaacaaaa ccaaagccac
      541 taagtcaagc tgaaatgagt cctgctgcat ctgcagccat cagaaatgat gtcgcaaagc
      601 aacgtgctgc accaaaagtc tctcctcatg ttaagcctga acagaaacag atggtggccc
      661 agcaggaatc tatcagagaa gggtttcaga agcgctgttt gccagttatg gtactgaaag
      721 caaagaagcc cttcacgttt gagacccaag aaggcaagca ggagatgttt catgctacag
      781 tggctacaga aaaggaattc ttctttgtaa aagtttttaa tacactgctg aaagataaat
      841 tcattccaaa gagaataatt ataatagcaa gatattatcg gcacagtggt ttcttagagg
      901 taaatagcgc ctcacgtgtg ttagatgctg aatctgacca aaaggttaat gtcccgctga
      961 acattatcag aaaagctggt gaaaccccga agatcaacac gcttcaaact cagccccttg
     1021 gaacaattgt gaatggtttg tttgtagtcc agaaggtaac agaaaagaag aaaaacatat
     1081 tatttgacct aagtgacaac actgggaaaa tggaagtact gggggttaga aacgaggaca
     1141 caatgaaatg taaggaagga gataaggttc gacttacatt cttcacactg tcaaaaaatg
     1201 gagaaaaact acagctgaca tctggagttc atagcaccat aaaggttatt aaggccaaaa
     1261 aaaaaacaga ggatccccgg gtaccggtcg ccaccatgga caacaccgag gacgtcatca
     1321 aggagttcat gcagttcaag gtgcgcatgg agggctccgt gaacggccac tacttcgaga
     1381 tcgagggcga gggcgagggc aagccctacg agggcaccca gaccgccaag ctgcaggtga
     1441 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct
     1501 ccaaggccta cgtgaagcac cccgccgaca tccccgacta catgaagctg tccttccccg
     1561 agggcttcac ctgggagcgc tccatgaact tcgaggacgg cggcgtggtg gaggtgcagc
     1621 aggactcctc cctgcaggac ggcaccttca tctacaaggt gaagttcaag ggcgtgaact
     1681 tccccgccga cggccccgta atgcagaaga agactgccgg ctgggagccc tccaccgaga
     1741 agctgtaccc ccaggacggc gtgctgaagg gcgagatctc ccacgccctg aagctgaagg
     1801 acggcggcca ctacacctgc gacttcaaga ccgtgtacaa ggccaagaag cccgtgcagc
     1861 tgcccggcaa ccactacgtg gactccaagc tggacatcac caaccacaac gaggactaca
     1921 ccgtggtgga gcagtacgag cacgccgagg cccgccactc cggctcccag tagagcggcc
     1981 gcgactctag aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa
     2041 aaataatagg cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     2101 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2161 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     2221 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     2281 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     2341 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2401 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2461 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2521 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2581 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2641 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2701 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     2761 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     2821 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     2881 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     2941 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3001 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3061 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3121 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3181 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     3241 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3301 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3361 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3421 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3481 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3541 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3601 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3661 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3721 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3781 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3841 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3901 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3961 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4021 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4081 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4141 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4201 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4261 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
//
