LOCUS       sequence-78766-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pCDM4-GLY
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggccacgatg cgtccggcgt agcctaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acaggaggca tatgtctgta attaagatga ccgatctgga
      181 tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa
      241 agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca
      301 aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg agtacaacga
      361 agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta acccggttcg
      421 tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg ttgttctgga
      481 aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa accctgtcca aaaaatacgc
      541 tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg cgcaggcttc
      601 tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga
      661 actggacgcg ctgggtaaag cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg
      721 tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa tcgctgacca
      781 gctgattgtt ggtggtggta tcgctaacac ctttatcgcg gcaca
//
