LOCUS       sequence-78774-e        8842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  p2attNG landing pad plasmid with 400 bp homology arms to the human 
            H11 locus (for use with TALEN-assisted recombination)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, 
            Chen-Tsai Y, Calos MP
  TITLE     DICE, an efficient system for iterative genomic editing in human 
            pluripotent stem cells.
  JOURNAL   Nucleic Acids Res. 2013 Dec 4.
  PUBMED    24304893
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Dec 4."
COMMENT     Sequence Label: p2attNG-H11-short
FEATURES             Location/Qualifiers
     source          1..8842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    61..82
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(97..102,103..120,121..127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    135..151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     140..162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     159..175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     159..175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        196..214
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    723..822
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     promoter        992..1096
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1098..1455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1098..1289
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1121..1141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1306..1441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1490..2284
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1490..2284
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1544..1563)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2154..2173
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2472..2491)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2516..2563
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        2835..3138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3140..3417
                     /label=chicken beta-actin promoter
     intron          3419..4436
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4360..4382
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4444..4463
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4514..4523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4520..4522,4523..4525,4526..5239)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4565..4586)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4826..4845)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5173..5194
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5361..5482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5398..5417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5452..5471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5646..5693
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     primer_bind     complement(6226..6245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6227..6245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6252..6269)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6252..6268)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6261..6283)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6410..6865
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6497..6516)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6707..6728
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6891..6995
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6996..7064,7065..7856)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7214..7233)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8027..8615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8516..8535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8769..8786
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
       61 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      121 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      181 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggagc tgatttaaat
      241 gactcagaac tgtactgtat tctgtagaat ctttatccgt aagtgctatg tatatactaa
      301 tagttaaggt gaataataaa acaattgtga gctaaggaat gtgatacaga acacaaggag
      361 tcaattaatg catgagtctt aagggaaatt cttagacttc agtgggtcta agctcagaac
      421 tctgagctta gagaaacagg atgccccagt aaacatgatt tgtttgagag aaactaccat
      481 atgtaataga tgattcctca ataaactgtt ttcactaggt cagtttaatc ttggaaatta
      541 cactatagat atcctggtat tagccacaag gctatgctat ctatagggtc agtccagtat
      601 aaactgacct gttggggtct ttaaagctca aggaaaaagg agatcttttc tcgactagta
      661 ctgacggaca caccgaagcc ccggcggcaa ccctcagcgg atgccccggg gcttcacgtt
      721 ttcccaggtc agaagcggtt ttcgggagta gtgccccaac tggggtaacc tttgagttct
      781 ctcagttggg ggcgtagggt cgccgacatg acacaagggg ttgtgaccgg ggtggacacg
      841 tacgcgggtg cttacgaccg tcagtcgcgc gagcgcgact agtcgagaag gatcctttgt
      901 cgacgaagtt cctattccga agttcctatt cttcaaaagg tataggaact tcgctagcga
      961 ttattcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1021 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1081 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1441 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     1501 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     1561 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     1621 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     1681 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     1741 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     1801 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     1861 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     1921 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     1981 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     2041 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     2101 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     2161 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     2221 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     2281 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     2341 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     2401 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
     2461 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
     2521 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
     2581 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
     2641 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
     2701 aacgtcgggg cggcaggccc tgccatagcc tcaggttact caggatcatc tctagaaaca
     2761 attctgcagg aatctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2821 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3121 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     3181 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     3241 ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
     3301 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     3361 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcgggg
     3421 agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     3481 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     3541 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     3601 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     3661 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     3721 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     3781 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     3841 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     3901 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     3961 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     4021 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     4081 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     4141 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     4201 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     4261 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     4321 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     4381 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     4441 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg atttgatacc
     4501 gcgggcccgg gccgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4921 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4981 ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acatcgagga
     5041 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5101 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5221 ggacgagctt tacaagtaag aattctagat aactgatcat aatcagccat accacatttg
     5281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5461 ccaaactcat caatgtatct tatttctaga gaagttccta ttccgaagtt cctattcttc
     5521 aaaaggtata ggaacttcaa gcttctaacc ccgcatacca agaaaccccc tacccggccc
     5581 gcgaaggcta ggtagggggc tttttgtgtt tcagtgggta tggccgtgat gacctgtgtc
     5641 ttcgtggttt gtctggtcaa ccaccgcggt ctcagtggtg tacggtacaa acccatgaga
     5701 gccctggtag tcatccgcct gtcccgcgtc accgatgcta cgacttcacc ggagcgtcag
     5761 ctggagtctt gccagcagct ctgcgcccag cgcggcggcc aattccatag ttgatttctc
     5821 ctaaatcaag atagagtcca attaactttt tttttttttt aagatggagt ctcactctgt
     5881 cgccaggctg gactgcagtg gcgtgatctc agctcactgc aacctctgcc tcccaggttc
     5941 aggcgattct tctgtctcag cctcccgagt agctgggact acaagtgtgg gccaccatgc
     6001 ccagctagtt ttgtattttt agtagagatg ggatttcacc atcttggcta ggatggtctt
     6061 gatctcttga ccttgtgatt cgcctgcctc tgcctcccag agtgctgaga ttacaggcgt
     6121 gagccacagt gcccaatttc catgggtttt caagaaaaac ttaacttact taaacttcag
     6181 atcacttcct taaaggaaca tactggcgcg cctgtaccca attcgcccta tagtgagtcg
     6241 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6301 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6361 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6421 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6481 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6541 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6601 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6661 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6721 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6781 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6841 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6961 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7081 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7141 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7201 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7261 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7321 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7381 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7441 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7501 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7561 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7621 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8161 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8821 tccccgcgcg ttggccgatt ca
//
