LOCUS       sequence-78784-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick vector for pathway engineering in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Xu P, Vansiri A, Bhan N, Koffas MA
  TITLE     ePathBrick: a synthetic biology platform for engineering metabolic 
            pathways in E. coli.
  JOURNAL   ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. 
            Epub 2012 May 4.
  PUBMED    23651248
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300016b";
            journalName: "ACS Synth Biol"; date: "2012-07-20- 20"; volume: "1"; 
            issue: "7"; pages: "256-66"
COMMENT     Sequence Label: pCOM4
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(45..136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(179..814)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(324..343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt cctttttcaa
       61 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      121 tagaaaaata aacaaatagg catgcaagcg cagaaaccaa ataggcatgc aagcgcagaa
      181 acgtcctaga agatgccagg aggatactta gcagagagac aataaggccg gagcgaagcc
      241 gtttttccat aggctccgcc cccctgacga acatcacgaa atctgacgct caaatcagtg
      301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctgatg gctccctctt
      361 gcgctctcct gttcccgtcc tgcggcgtcc gtgttgtggt ggaggcttta cccaaatcac
      421 cacgtcccgt tccgtgtaga cagttcgctc caagctgggc tgtgtgcaag aaccccccgt
      481 tcagcccgac tgctgcgcct tatccggtaa ctatcatctt gagtccaacc cggaaagaca
      541 cgacaaaacg ccactggcag cagccattgg taactgagaa ttagtggatt tagatatcga
      601 gagtcttgaa gtggtggcct aacagaggct acactgaaag gacagtattt ggtatctgcg
      661 ctccactaaa gccagttacc aggttaagca gttccccaac tgacttaacc ttcgatcaaa
      721 ccgcctcccc aggcggtttt ttcgtttaca gagcaggaga ttacgacgat cgtaaaagga
      781 tctcaagaag atcctttacg gattcccgac accatcactc tagatttcag tgcaattt
//
