LOCUS       sequence-78789-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for membrane PIP3 levels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR SFFVp PHAkt Cerulean
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(75..788,789..791,792..794))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(120..141)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     469..488
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     728..749
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atccagaggt tgattatcga taagcttgat atcaagcttg catgcctgca ggtcgactct
       61 agagtcgcgg ccgcttactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
      121 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac
      181 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
      241 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
      301 ttggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtc gctgatggcg
      361 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
      421 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
      481 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
      541 aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga agcactgcac gccccaggtc
      601 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
      661 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
      721 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
      781 cccttgctca ccattcttgg ccccatatgg ccgctaggta ccttgccgaa a
//
