LOCUS       sequence-78790-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a fragment containing 429 bp of the human HSPA5 promoter (-282 to 
            +147).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, 
            Conaway RC
  TITLE     Endoplasmic reticulum stress-responsive transcription factor 
            ATF6alpha directs recruitment of the Mediator of RNA polymerase II 
            transcription and multiple histone acetyltransferase complexes.
  JOURNAL   J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 
            10.1074/jbc.M112.369504. Epub 2012 May 10.
  PUBMED    22577136
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.369504"; journalName: "J Biol Chem"; date: 
            "2012-06-29- 29"; volume: "287"; issue: "27"; pages: "23035-45"
COMMENT     Sequence Label: pGEM-HSPA5P
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(531..549)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(532..549)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(567..583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(567..583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(580..602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    591..607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(615..621,622..639,640..645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    660..681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(798..815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggccgcg ggaattcgat ttagacagct gctgaaccaa tgggaccagc ggatggggcg
       61 gatgttatct accattggtg aacgttagaa acgaatagca gccaatgaat cagctggggg
      121 gggcggagca gtgacgttta ttgcggaggg ggccgcttcg aatcggcggc ggccagcttg
      181 gtggcctggg ccaatgaacg gcctccaacg agcagggcct tcaccaatcg gcggcctcca
      241 cgacggggct gggggagggt atataagccg agtaggcgac ggtgaggtcg acgccggcca
      301 agacagcaca gacagattga cctattgggg tgtttcgcga gtgtgagagg gaagcgccgc
      361 ggcctgtatt tctagacctg cccttcgcct ggttcgtggc gccttgtgac cccgggcccc
      421 tgccgcctgc aagtcggaaa ttgcgctgtg aatcactagt gaattcgcgg ccgcctgcag
      481 gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt
      541 cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
      601 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
      661 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
      721 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      781 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      841 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
//
