LOCUS       sequence-78791-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic PI3K activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR iSH-YFP-PIF
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(390..641)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(432..453)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     781..800
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttgtaatcc agaggttgat tatcgataag cttgatatca agcttgcatg cctgcaggtc
       61 gactctagag tcgcggccgc ttagtcaaca tgtttattgc tttccaacat gtttgtttca
      121 tgggcagcag caacatgact ttgtggaaca acctgcgtgt ccccgagatt tgtgatggca
      181 ccaccaccac catggatgat actcttcatg aaatcctcgt tatactctgc ctcatacaaa
      241 tccatgatcg atttggtacg ttgattatgc agagaaacgt tggatctttg gccctttgca
      301 agaatctggc cattctcaaa cacaagctcc atatactctt gatcgcttaa cctgcaacaa
      361 taatcggttg gtaagaacat catactagtc ttgtacagct cgtccatgcc gagagtgatc
      421 ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg
      481 ctcagcttgg actggtagct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg
      541 atgggggtgt tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg
      601 cggatcttga agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg
      661 tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg
      721 atgcccttca gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg
      781 gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcggg
//
