LOCUS       sequence-78792-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic PI3K activation system; cytoplasmic component.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Toettcher JE, Gong D, Lim WA, Weiner OD
  TITLE     Light-based feedback for controlling intracellular signaling 
            dynamics.
  JOURNAL   Nat Methods. 2011 Sep 11;8(10):837-9. doi: 10.1038/nmeth.1700.
  PUBMED    21909100
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-09-11- 11"; volume: "8"; 
            issue: "10"; pages: "837-9"
COMMENT     Sequence Label: pHR iSH-YFP-PIF
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..352
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     289..310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(774..794)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
       61 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      121 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      181 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      241 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      301 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agactagtat
      361 gatgttctta ccaaccgatt attgttgcag gttaagcgat caagagtata tggagcttgt
      421 gtttgagaat ggccagattc ttgcaaaggg ccaaagatcc aacgtttctc tgcataatca
      481 acgtaccaaa tcgatcatgg atttgtatga ggcagagtat aacgaggatt tcatgaagag
      541 tatcatccat ggtggtggtg gtgccatcac aaatctcggg gacacgcagg ttgttccaca
      601 aagtcatgtt gctgctgccc atgaaacaaa catgttggaa agcaataaac atgttgacta
      661 agcggccgcg actctagagt cgacctgcag gcatgcaagc ttgatatcaa gcttatcgat
      721 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
      781 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
      841 at
//
