LOCUS       sequence-78794-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN401
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..71)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             151..159
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acttgtatgg aggcctgagc tcacttcttc ttcttcgcct gccccgcctt cttcgtcgcc
       61 gctggccgct tgtcgccccc gagctgagac aggtcgatgc gcgtctcgta gaggccggta
      121 atcgactggt ggatgagggt cgcgtccagg acctccttag tgcttgtgta cctcttgcga
      181 tcgatagttg tgtcgaagta cttgaaagca gcaggggcgc cgaggttcgt cagggtgaag
      241 agatgaatga tattctcagc ctgctccctg attggcttgt cgcggtgctt gttgtacgcg
      301 gagaggacct tatccagatt cgcgtcggcc aggatcacgc gcttggagaa ctcggaaatc
      361 tgctcaatga tctcgtcgag gtaatgcttg tgctgctcga cgaacagctg cttctgctcg
      421 ttgtcctcgg ggctgccctt gagcttctcg tagtgggagg ccaggtagag gaagttcaca
      481 tacttggacg gcagagccag ctcgttcccc ttctgcagct cgccagcgga agccagcatc
      541 cgcttcctgc cgttctccag ctcgaagagt gagtacttgg ggagcttaat gatcaggtcc
      601 ttcttcacct ccttgtagcc cttcgcctcc aggaaatcga tcgggttctt ctcgaagctg
      661 gagcgctcca taatcgtgat ccccagcagc tccttcacgc tcttgagctt cttggacttg
      721 cccttctcaa ccttcgccac aaccaggacc gagtaggcca cagtggggct gtcgaacccg
      781 ccgtacttct tcggatccca gtccttcttg cgggcgatga gcttgtcgct gttccgctta
      841 ggcagaattg actccttaga gaacccgcca gtctggacct ctgtcttctt gacgatattc
      901 acttgtggca tggagagaac cttcctgacg gtcgcgaaat ccctgccctt gtcccacacg
      961 atctcccc
//
