LOCUS       sequence-78802-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN6A01
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(56..205)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(212..232)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(248..268)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(287..334)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             414..422
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 agtcgggcac atcgtatggg tatgagccaa ccttccgctt cttcttaggc gaccccagca
       61 tatcgaggtc aaaatcgtcc agagcgtccg acccgagcat atcgaggtca aagtcgtcga
      121 gcgcgtcgct cccgagcata tcgaggtcga agtcgtccag ggcgtcggag cccagcatat
      181 cgaggtcgaa atcgtcgagg gcgtccgacc cgaccttcct cttcttcttg gggctgccgc
      241 tgctgccgac cttcctcttc ttcttagggc tgcctgatga cccggacttc ttcttcttcg
      301 cctgccccgc cttcttcgtc gccgctggcc gcttgtcgcc cccgagctga gacaggtcga
      361 tgcgcgtctc gtagaggccg gtaatcgact ggtggatgag ggtcgcgtcc aggacctcct
      421 tagtgcttgt gtacctcttg cgatcgatag ttgtgtcgaa gtacttgaaa gcagcagggg
      481 cgccgaggtt cgtcagggtg aagagatgaa tgatattctc agcctgctcc ctgattggct
      541 tgtcgcggtg cttgttgtac gcggagagga ccttatccag attcgcgtcg gccaggatca
      601 cgcgcttgga gaactcggaa atctgctcaa tgatctcgtc gaggtaatgc ttgtgctgct
      661 cgacgaacag ctgcttctgc tcgttgtcct cggggctgcc cttgagcttc tcg
//
