LOCUS       sequence-78808-e       13237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively express bud neck localized PhyB in Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13237)
  AUTHORS   Yang X, Jost AP, Weiner OD, Tang C
  TITLE     A light-inducible organelle-targeting system for dynamically 
            activating and inactivating signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 
            10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.
  PUBMED    23761071
REFERENCE   2  (bases 1 to 13237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0126"; journalName: "Mol Biol Cell"; date: 
            "2013-08"; volume: "24"; issue: "15"; pages: "2419-30"
COMMENT     Sequence Label: PhyB-mCherry-CDC10
FEATURES             Location/Qualifiers
     source          1..13237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6786..7490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6928..6946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7196..7215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(9784..9803)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9785..9803)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9810..9827)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9810..9826)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9819..9841)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9967..10422
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10054..10073)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10264..10285
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10778..10797)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10897..10919
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10957..10975)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11060..11164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11165..11233,11234..12025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11383..11402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12196..12784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12685..12704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12938..12955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13072..13093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13108..13113,13114..13131,13132..13138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13146..13162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13151..13173
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13170..13186
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13170..13186
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        13207..13225
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaaa gcaatggttt
     4021 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     4081 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     4141 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     4201 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     4261 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     4321 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     4381 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     4441 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     4501 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     4561 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     4621 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     4681 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     4741 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     4801 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     4861 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt
     4921 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     4981 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     5041 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     5101 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     5161 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     5221 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     5281 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     5341 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     5401 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     5461 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     5521 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     5581 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     5641 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     5701 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     5761 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     5821 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg
     5881 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg
     5941 aacaggggat tgatgagtta ggtgcagttg caagagagat ggttaggctc attgagactg
     6001 caactgttcc tatattcgct gtggatgccg gaggctgcat caatggatgg aacgctaaga
     6061 ttgcagagtt gacaggtctc tcagttgaag aagctatggg gaagtctctg gtttctgatt
     6121 taatatacaa agagaatgaa gcaactgtca ataagcttct ttctcgtgct ttgagagggg
     6181 acgaggaaaa gaatgtggag gttaagctga aaactttcag ccccgaacta caagggaaag
     6241 cagtttttgt ggttgtgaat gcttgttcca gcaaggacta cttgaacaac attgtcggcg
     6301 tttgttttgt tggacaagac gttactagtc agaaaatcgt aatggataag ttcatcaaca
     6361 tacaaggaga ttacaaggct attgtacata gcccaaaccc tctaatcccg ccaatttttg
     6421 ctgctgacga gaacacgtgc tgcctggaat ggaacatggc gatggaaaag cttacgggtt
     6481 ggtctcgcag tgaagtgatt gggaaaatga ttgtcgggga agtgtttggg agctgttgca
     6541 tgctaaaggg tcctgatgct ttaaccaagt tcatgattgt attgcataat gcgattggtg
     6601 gccaagatac ggataagttc cctttcccat tctttgaccg caatgggaag tttgttcagg
     6661 ctctattgac tgcaaacaag cgggttagcc tcgagggaaa ggttattggg gctttctgtt
     6721 tcttgcaaat cccgagcgaa ttcgatagtg ctggtagtgc tggtagtgct ggtggtagtt
     6781 ccctagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcgata
     7501 gcgcaggcag tgctggtaag gctagcatgg atcctctcag ctcagtacag cctgcttctt
     7561 atgttggttt tgataccatc acgaatcaga tcgaacatcg tctgttgaag aaaggttttc
     7621 aatttaatat aatggttgtt ggccaatccg gattgggtaa aagtactcta ataaatacgt
     7681 tatttgcctc acatttgatt gattctgcta ctggtgatga tatttctgcc ctgcctgtta
     7741 caaaaacaac tgaaatgaaa atttctactc atactcttgt ggaggaccgc gttcgcttga
     7801 atattaatgt tatagataca cctggatttg gtgactttat tgacaattct aaagcttggg
     7861 agcctattgt gaagtacatt aaggaacaac attctcaata cttacgtaaa gaattgacag
     7921 cccaacgtga aaggtttatt actgatacaa gagttcatgc aattctttat ttcctgcaac
     7981 caaatggaaa ggagttgagc cgccttgacg ttgaagcctt gaaaagattg acagaaatag
     8041 caaatgttat accagttatt ggcaagtcgg atacattgac tttagatgaa agaacggagt
     8101 ttagggagct tattcaaaat gaattcgaaa aatacaattt caagatttat ccttatgatt
     8161 cggaagaact aactgacgag gaattagaac taaacagaag tgttagatct atcattccgt
     8221 ttgcagtggt tggttctgag aatgagattg aaataaacgg tgaaaccttc aggggaagaa
     8281 aaactcgttg gagcgctatt aatgttgagg atatcaacca gtgtgatttt gtatatttaa
     8341 gggaattttt gattcgaact catctccaag acttaatcga aacaacttcc tacattcatt
     8401 atgaagggtt cagagcaaga caattaattg ccttgaaaga aaatgcgaat agtcgttcct
     8461 cagctcatat gtctagcaac gccattcaac gttgagcggc cgccaccgcg gtggagctct
     8521 aagcaaatag ctaaattata tacgaattaa tattatgatt aagtgtttac gtgagtgcga
     8581 tatttttatt actatcttat acagttgtat atactctata aaatgagttg tctattaatt
     8641 aacgcgatga atgctttctg ggtttacctc tccaacaact ctagtttact tctcaataca
     8701 ttcaattgta tttgatttgt caatacttca tcattaatca attctatagt tttgtttttc
     8761 tcgtttattt ccaaatttaa tgcatcaatt ttattattca atttgtcgtt gattttggtt
     8821 aatgatttta tggtttgatc tctggcattg attgtttgtg ttagtttttc attattgata
     8881 attaaattat ttaagttagt tatcaactcg gtgttttcaa gtttcaagtt ttcaatttct
     8941 ttagagttta ttagatttgt caaagtttct gaattgcttg attggtcctg tagaagagta
     9001 tttgttgttg tggataattg attcaatttt tgagacaatt gctggaaggc gttgaaatat
     9061 ctagcatcaa tctcatggtt tttttcccga gagtctcgta gattcaattg ttttaatata
     9121 tcttgggacc actcttgatt tgaactcatg gaaattaaac tgggtgttgt gttgtggtgt
     9181 aatgattgta ccccctttgc ttataattgt gtggccaccg aagtcggtga tgctgtcgcc
     9241 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     9301 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     9361 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     9421 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
     9481 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
     9541 acagaaaatc atgaaactat gattcctaat ttatatattg gaggattttc tctaaaaaaa
     9601 aaaaaataca acaaataaaa aacactcaat gacctgacca tttgatggag tttaagtcaa
     9661 taccttcttg aaccatttcc cataatggtg aaagttccct caagaatttt actctgtcag
     9721 aaacggcctt aacgacgtag gccggccatt taaatgttta aacggccacg ctggcccaat
     9781 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     9841 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9901 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9961 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    10021 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    10081 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    10141 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    10201 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    10261 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    10321 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    10381 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
    10441 ccttacgcat ctgtgcggta tttcacaccg catagggtaa taactgatat aattaaattg
    10501 aagctctaat ttgtgagttt agtattagga tctctaccct tggcgaaaag tcctctgcca
    10561 acaatgatga tatctgatcc accacttaca acttcgtcga cggttctgta ctgctgaccc
    10621 aatatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg
    10681 gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa
    10741 aatgatgaat tgaaaaggtg gtatggtgca ctctcagtac aatctgctct gatgccgcat
    10801 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    10861 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    10921 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
    10981 aggttaatgt catgataata atggtttctt agacgtcggc gcgccgagga cgtcaggtgg
    11041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    11101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    11161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    11221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    11281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    11341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    11401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    11461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    11521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    11581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    11761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    11821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    11881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    11941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    12001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    12061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    12121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    12181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    12241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    12301 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    12361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    12421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    12481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    12541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    12601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    12661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    12721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    12781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    12841 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    12901 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    12961 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    13021 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    13081 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg
    13141 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    13201 gctcggaatt aaccctcact aaagggaaca aaagctg
//
