LOCUS       sequence-78809-e         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gateway adapted mammalian expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang 
            X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, 
            Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz
            G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, 
            Meyerson M
  TITLE     Functional analysis of receptor tyrosine kinase mutations in lung 
            cancer identifies oncogenic extracellular domain mutations of ERBB2.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug
            20.
  PUBMED    22908275
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-4"; volume: "109(36)"; pages: 
            "14476-81. Epub 2012 Aug 20"
COMMENT     Sequence Label: pBABEpuro-gateway
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(270..290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        275..604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      455..590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(614..633)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatgtag
      241 gaattcgcca gcacagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc
      301 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tangcttttg
      601 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
      661 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
      721 c
//
