LOCUS       sequence-78810-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human eIF2C1 mutant in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pcDNAM-human eIF2C1-A-Myc
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..26
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(61..90)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gctggcacta gaaggcacag tcgaggctga tcagcgggtt tattatcagg tatgcatatt
       61 cagatcctct tctgagatga gtttttgttc gaaccgcggg ccctctagat gcatgctcga
      121 gcggccgcga ctgatctcct cctgtctgtc tggagcggat ctagctgtgg cctttatcat
      181 ggtcgaggtc tggttgtcgg tcagcttttt aatacagcgc tgcccagcca caatgttaca
      241 gacctctagg ggaaggtagg tatgcttttg ttcctggcca acttgtaggc agggcagatg
      301 gggatacttg agctgaaggt tatatttctg cttgaaatac tgtgccactg tgcactccac
      361 agtctgtcca ctctccagct gtaaggggaa tgtctgatgg ctagcagggc gacgggtaac
      421 attacacacg cggtacttcc tcttcatctg tccacagtgg gtgacttcca ccttcaggcc
      481 cttgatctcc ttggtgaagc gaacgcgctg agagtccgtg aggggcttgg gctgctcatc
      541 tatgttcctg atgtccagca cctcacacat gaactcaatc actggctgtg ccttataaaa
      601 ggcagtggct gagacatcaa tgttgagcat catcttccac atggcagggc gcacagactg
      661 gtgaaagccg aaccagacct cgcgcccacc ccccagcggg tggtagtagc cctcaggcgg
      721 tgagaagaag gagcggccca caggggtgta cctcatggat gccaggtgcc tcatggccac
      781 atccagggct tgcacagact ccaagggaac agggatctgg ccgctgacca gggcctcatg
      841 cagcattcgc cagctc
//
