LOCUS       sequence-78812-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human eIF2C1 mutant in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Doi N, Zenno S, Ueda R, Ohki-Hamazaki H, Ui-Tei K, Saigo K
  TITLE     Short-interfering-RNA-mediated gene silencing in mammalian cells 
            requires Dicer and eIF2C translation initiation factors.
  JOURNAL   Curr Biol. 2003 Jan 8;13(1):41-6.
  PUBMED    12526743
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr 
            Biol."; date: "2003-01-8"; volume: "13(1)"; pages: "41-6"
COMMENT     Sequence Label: pcDNAM-human eIF2C1-B-Myc
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..27
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(62..91)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggctggcact agaaggcaca gtcgaggctg atcagcgggt ttattatcag gtatgcatat
       61 tcagatcctc ttctgagatg agtttttgtt cgaaccgcgg gccctctaga tgcatgctcg
      121 agcggccgcc agcgaagtac atggtgcgca gagtatcctg gtgaacctgc acggctttgg
      181 ccagggcctg ggggtcccgc ccattgctct gccccgatat gtggctcccc tctccactgt
      241 catgctcctt gtccaccagg tggtatcgtg cccggaaagc caccaggcgg gcatagtagg
      301 caggtgctgg gatagagaca gagcgtgtgc atcgtacgta agtgtggcac agctggtacg
      361 tcaggatctg gagctcatct gctgtgaaac ggttgtcatc ccaaagaaca tagtaatggg
      421 atggtcggct ggtgccctgg atgcctgcgt ggctgcacag atagaagtca aactcaaatg
      481 ggtgggtgat gttggtgtcc actgtggtcc cagctgggat gttaccactc ttcccaattc
      541 gctcattctt gtcagcacag aaaaggcggg tgtgatggcg tttctgcacc acaatataag
      601 tgataccagg ctggtagtcc ttttccagtt tgatgcaggc atcacgaatg gccagtagct
      661 catagtggag tatctggggt agctggcctt caggcacccc atctcggtag aagatgatgc
      721 gggtaggctt gaaacgggtg gacttgtaga attggatgag gagctcacgc accatgtagg
      781 acaagtcttc aaggatctct tgccgtggtc gctgtacccg cacagtagca cagtatcggc
      841 tggggtgggc atccatactg cctaccactg ctgtgataga aggttttttc ccatcccctg
      901 ctggggggtg tgtaacatct gctcccagga atatcactgg ctgttgaaaa acgg
//
