LOCUS       sequence-78817-e       18054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively express myosin ring localized PhyB in Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Yang X, Jost AP, Weiner OD, Tang C
  TITLE     A light-inducible organelle-targeting system for dynamically 
            activating and inactivating signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 
            10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.
  PUBMED    23761071
REFERENCE   2  (bases 1 to 18054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0126"; journalName: "Mol Biol Cell"; date: 
            "2013-08"; volume: "24"; issue: "15"; pages: "2419-30"
COMMENT     Sequence Label: PhyB-mCherry-MYO1
FEATURES             Location/Qualifiers
     source          1..18054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6786..7490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6928..6946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7196..7215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(14601..14620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14602..14620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14627..14644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14627..14643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14636..14658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14784..15239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14871..14890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15081..15102
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(15595..15614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15714..15736
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(15774..15792)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        15877..15981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15982..16050,16051..16842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16200..16219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17013..17601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17502..17521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17755..17772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    17889..17910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17925..17930,17931..17948,17949..17955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17963..17979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17968..17990
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17987..18003
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17987..18003
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        18024..18042
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaaa gcaatggttt
     4021 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     4081 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     4141 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     4201 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     4261 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     4321 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     4381 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     4441 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     4501 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     4561 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     4621 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     4681 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     4741 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     4801 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     4861 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt
     4921 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     4981 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     5041 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     5101 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     5161 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     5221 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     5281 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     5341 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     5401 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     5461 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     5521 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     5581 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     5641 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     5701 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     5761 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     5821 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg
     5881 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg
     5941 aacaggggat tgatgagtta ggtgcagttg caagagagat ggttaggctc attgagactg
     6001 caactgttcc tatattcgct gtggatgccg gaggctgcat caatggatgg aacgctaaga
     6061 ttgcagagtt gacaggtctc tcagttgaag aagctatggg gaagtctctg gtttctgatt
     6121 taatatacaa agagaatgaa gcaactgtca ataagcttct ttctcgtgct ttgagagggg
     6181 acgaggaaaa gaatgtggag gttaagctga aaactttcag ccccgaacta caagggaaag
     6241 cagtttttgt ggttgtgaat gcttgttcca gcaaggacta cttgaacaac attgtcggcg
     6301 tttgttttgt tggacaagac gttactagtc agaaaatcgt aatggataag ttcatcaaca
     6361 tacaaggaga ttacaaggct attgtacata gcccaaaccc tctaatcccg ccaatttttg
     6421 ctgctgacga gaacacgtgc tgcctggaat ggaacatggc gatggaaaag cttacgggtt
     6481 ggtctcgcag tgaagtgatt gggaaaatga ttgtcgggga agtgtttggg agctgttgca
     6541 tgctaaaggg tcctgatgct ttaaccaagt tcatgattgt attgcataat gcgattggtg
     6601 gccaagatac ggataagttc cctttcccat tctttgaccg caatgggaag tttgttcagg
     6661 ctctattgac tgcaaacaag cgggttagcc tcgagggaaa ggttattggg gctttctgtt
     6721 tcttgcaaat cccgagcgaa ttcgatagtg ctggtagtgc tggtagtgct ggtggtagtt
     6781 ccctagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcgata
     7501 gcgcaggcag tgctggtaag gctagcatga ccggcgggca gtcttgcagt tctaatatga
     7561 tcgtctggat accagatgag aaagaagttt tcgcaaaagg ggagctgatg agtaccgata
     7621 tcaacaagaa taaatttaca ggccaagaag aacaaatagg aattgtccac ccgttggatt
     7681 ccacggaggt ttctaatcta gtgcaggtac gaatatccga tgtatttcca gttaacccat
     7741 cgacttttga caaagtagag aatatgtctg agttaaccca tttgaatgag ccgtctgtgc
     7801 tttataacct agaaaagagg tacgattgtg atctaatata cacctattcc ggcttatttt
     7861 tagtggcgat taacccttat cacaatttga atttatattc tgaagaccat ataaacttgt
     7921 accacaacaa gcataacagg ttatcaaaga gcagcttgga tgaaaactcc catgaaaaac
     7981 tgccgccgca tatatttgca attgcagaag aagcctatga gaatctttta tctgaaggaa
     8041 aagatcaatc tattttagtc actggtgaat ccggtgcggg caagacagaa aatacgaaaa
     8101 agattctaca atatctagca tctataactt ctggctctcc ttccaatata gctcctgtta
     8161 gtggtagttc tattgtagaa agcttcgaaa tgaaaattct acaaagtaac cctatcttag
     8221 agtcttttgg taatgcacag actgtacgaa ataacaactc ttcaagattc ggtaaattca
     8281 taaagataga atttaatgaa catggtatga tcaatggtgc gcatatcgag tggtaccttt
     8341 tagagaaatc aagaattgtt catcaaaatt cgaaagaaag aaattatcat atattttacc
     8401 aactattatc cggtttagac gattctgagt tgaaaaatct acgccttaaa tctaggaacg
     8461 taaaagatta caaaatttta tccaattcta accaggatat tataccagga atcaacgacg
     8521 tagagaattt taaagaactg ctctcggcat tgaatattat tgggttttca aaagatcaaa
     8581 taagatggat atttcaagta gtggcaatta ttttattaat cggtaacatt gagttcgtat
     8641 cagacagagc agaacaagca tctttcaaaa atgatgttag cgcaatttgt agcaatttag
     8701 gcgtggacga aaaagatttc caaactgcca tattaaggcc tagatcaaaa gccggaaaag
     8761 agtgggtttc acagtccaaa aactctcaac aagctaagtt catcttgaat gccttatcaa
     8821 gaaatcttta tgagcggttg ttcggatata ttgtggatat gattaataaa aacttggacc
     8881 atgggagtgc aactttgaat tacattggat tgttggatat tgctggtttt gaaatattcg
     8941 aaaataattc ctttgaacaa ttatgcatca actatacaaa tgaaaaatta cagcagttct
     9001 ttaataacca tatgtttgtt ttagaacaga gcgaatattt aaaggaaaac attcaatggg
     9061 attatataga ttacggaaaa gatttgcaac tgacgattga tttgatcgaa agcaaggggc
     9121 caccgaccgg tgtactaccg ttgttggatg aggaagccgt tttgcccaaa tccactgatg
     9181 agtcattcta ctctaaactg atctcaactt gggaccaaaa ctcttcaaag tttaaacgtt
     9241 caagattaaa aaatgggttc attttgaaac actatgctgg ggatgtggaa tacactgtgg
     9301 aaggctggtt atccaaaaac aaagatcctt taaacgataa tctcttgtct ttgttgtctt
     9361 cttcacaaaa cgatatcatt tcaaaactgt tccagccaga gggcgaaaaa tcttctagtg
     9421 ctggtgtgga agccaacatc tccaaccaag aagttaagaa atcagctagg acaagtacct
     9481 tcaagactac atcatcacgc catagagaac agcagataac attactaaat caactagctt
     9541 ctacccaccc acattttgtt cgttgtataa ttccaaacaa cgtgaaaaaa gtgaaaacat
     9601 ttaacagaag gttaatctta gatcaattac gttgtaatgg tgtgctagag ggtattagac
     9661 ttgccagaga aggttaccca aataggatag cattccaaga atttttccag cggtatagga
     9721 tcttgtatcc tgaaaattca accaccacga ctttcagttc taaattaaaa gccagtacca
     9781 aacaaaactg tgagtttctt ctaacgtctt tgcaactgga tacaaaagtt tataaaattg
     9841 gtaatactaa actgtttttc aaagctggag tattggcaga tttggaaaaa caaaaagatg
     9901 ttaagctgaa taatattatg attaaactaa cagcaactat acgaggttac acagtaagaa
     9961 aagaaataac gtaccatcta caaaaattaa agaaaacaag ggtgattggt aataccttca
    10021 gattatacaa tagactggtg aaggaagatc cttggtttaa tttatttatc aggatcaagc
    10081 cacttttaac atcatccaat gacatgacca gaaccaaaaa attcaacgag caaattaata
    10141 aactgaagaa cgaccttcaa gaaatggaat ctaagaagaa gtttttggaa gaaaagaacc
    10201 aaaaaactgt caatgagttg gaaaatacgc aggacttgct aaatcaggag aaagagaatt
    10261 taagaaaaaa tgagagctta ttaaatagag ttaaaaccag ctcagaaaca ttacaaaaac
    10321 aattcgatga ccttgtttct gaaaaggatg aaattagcag ggaaaaacta gaagttgcgc
    10381 aaaatcttga agaagctcat caaaaaatcc aaggccttca agaaactatt agagaacggg
    10441 aggcgacctt agagaaatta cactcaaaaa ataacgaact aataaagcaa atatcagact
    10501 tgaactgtga catttctaag gaacagagct cccagtcttt aatcaaagaa tctaaattga
    10561 agctagaaaa cgaaataaag agattaaaag atgtcataaa ctcgaaggag gaagaaataa
    10621 aatcgttcaa cgataagtta agttcctcag aagaagatct tgacataaaa ttggtcactt
    10681 tagagaaaaa ttgtaacatt gcgatgtcaa gactacaatc ccttgtaaca gaaaattcag
    10741 atttgcgttc gaaaaatgag aatttcaaga aagaaaaggc agcactgaat aaccagttga
    10801 aaaataagga aagtgaactg ttaaagatga aagaaaaaat agataatcac aaaaaagagc
    10861 ttgctacttt ttccaagcaa agagatgacg cagttagcga acatggtaaa attactgctg
    10921 aactaaaaga gacaagaatc caacttaccg agtataaatc taactatcaa aagattaaag
    10981 aagaatattc caacttccaa agagaaacga aggaacagga gcaaaagaaa agaaatagcc
    11041 tggttgagtc tctgaacgat agtaaaatta aagaattgga agctcggttg tcacaggaaa
    11101 tatccttaaa tcaatactta aacaaaagaa tatcaggaaa ttctgtagaa acgaatattt
    11161 cgtctactag acgttccaca tcgtattcag atgatccact tgacaaagaa gatataataa
    11221 aaaaatacta tgaccttcaa ttagcgttta ctgaaataac taggaatcta gagaatgaaa
    11281 ttgaagagaa gaagaactta atttctagat tgagattcac tgaaacaaga ctagcatctt
    11341 cgtcttttga ggaccaaaag attaaggcac aaatgaagaa attaaaaaaa ttgatccagg
    11401 atatggaccc tagtattcct ttggacagta ttctaaatga gccgctagat aactgccctg
    11461 acaaagagtc tgatattaac aaattaatgc ttgaggtcga ttatttaaaa agacaattgg
    11521 atatcgaaac aagagctcac tacgatgcag aaaatgccat atctgcttta cacagtaaat
    11581 ttagaaagat ccaaggggaa agctccctgt catcttctga tatttacaaa ctgaagttcg
    11641 aagccagtga agaaagagtc aaatccttgg aagacaagct aaaaaccatg cctttacgtg
    11701 atcgaacaaa tttacctgtc ggagatatta taaagaaccg tgatagcatt tcaaaatatg
    11761 aagaagaaat tcgctattat aaacttgaaa actacaagct ccaagaaata ttaaatgaat
    11821 caaatggaaa attgagccaa ctcactcttg acttgaggca atcaaaatcc aaagaagccc
    11881 tacttagtga acaattagac agattacaga aagaccttga atctacggaa cgccaaaaag
    11941 aactactatc atccacaata aaacagcaga aacagcaatt tgaaaactgc atggacgatc
    12001 tgcaagggaa tgaactgcgg ctaagagaac atattcatgc actaaaacaa gctgaagagg
    12061 acgtaaagaa tatggcgtct atcattgaaa aattgaaaac tcaaaataag caaaaggaaa
    12121 agttaatctg ggagcgcgaa atggaaagaa atgactcaga tatgcaatta caggaaactc
    12181 tgctggagtt gaagagagtc caagatgtaa agaaaatcct gagtgatgat ttggcccatt
    12241 taaaggagcg attgagcgct gtagaagata gatcccaata tacggatgag attaatagat
    12301 taaaggaaga attgaactgc tctttaaaag ctgaaacaaa cttaaaaaaa gaatttgcaa
    12361 cccttaagta caaactggaa acttcgacta atgattctga agcaaaaatc tccgatctgc
    12421 tgaagcagct cgatcattat acaaaagtgg tggaaatgtt gaacaacgaa aaagatgcta
    12481 tttctcttgc agaaaaagaa ctttatcaga aatacgaggc actcaatact gaatgcgagt
    12541 ctcttaaagg aaagatagtg tctttgacta aaatcaagca ggaactggaa tctgacttaa
    12601 atcaaaagac tgatgcgcta cagatatcaa acgcagcact ctcaagttcc acacaaaaga
    12661 ataaagagat tactgaaaaa atcaaatatc tggaggaaac actacaactg caaatggaac
    12721 aaaattcaag gaatggggag ttggttaaga cattacaggc cagctgcaat ggatataaag
    12781 ataagtttga tgatgagaag cagaagaata ttgatttata tgaagaaaat caaactttac
    12841 aaaagctcaa caccgactta cagcttcaac taaaaaattt gcatgaaaga ttatcagaca
    12901 ctactgaaaa aaacgcatgg ttatcaaaga ttcatgaatt ggaaaatatg gtaagcctag
    12961 aaacggactt gaaatatgaa gaaatgaaaa agaacaagag tttggagaga gcagtggaag
    13021 agttacaaac taagaactcc caacaaacag atgtaataga actagcgaat aaaaatagaa
    13081 gcgaatttga ggaagctact ttgaaatatg aggctcaaat ctctgactta gaaaagtaca
    13141 tttctcaaca agagcttgag atgaagaaat ccattagaga taattcttct taccgcgaca
    13201 aagtgcagga aatggcccaa gaaattgaat tctggaaaag ccgttatgaa tctaccatga
    13261 taggctcgaa aaatattgat agtagcaatg cacagagtaa aatttcagtt aagcggccgc
    13321 caccgcggtg gagctctaag caaatagcta aattatatac gaattaatat tatgattaag
    13381 tgtttacgtg agtgcgatat ttttattact atcttataca gttgtatata ctctataaaa
    13441 tgagttgtct attaattaac gcgatgaatg ctttctgggt ttacctctcc aacaactcta
    13501 gtttacttct caatacattc aattgtattt gatttgtcaa tacttcatca ttaatcaatt
    13561 ctatagtttt gtttttctcg tttatttcca aatttaatgc atcaatttta ttattcaatt
    13621 tgtcgttgat tttggttaat gattttatgg tttgatctct ggcattgatt gtttgtgtta
    13681 gtttttcatt attgataatt aaattattta agttagttat caactcggtg ttttcaagtt
    13741 tcaagttttc aatttcttta gagtttatta gatttgtcaa agtttctgaa ttgcttgatt
    13801 ggtcctgtag aagagtattt gttgttgtgg ataattgatt caatttttga gacaattgct
    13861 ggaaggcgtt gaaatatcta gcatcaatct catggttttt ttcccgagag tctcgtagat
    13921 tcaattgttt taatatatct tgggaccact cttgatttga actcatggaa attaaactgg
    13981 gtgttgtgtt gtggtgtaat gattgtaccc cctttgctta taattgtgtg gccaccgaag
    14041 tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc ttaaaaagat tctctttttt
    14101 tatgatattt gtacataaac tttataaatg aaattcataa tagaaacgac acgaaattac
    14161 aaaatggaat atgttcatag ggtagacgaa actatatacg caatctacat acatttatca
    14221 agaaggagaa aaaggaggat gtaaaggaat acaggtaagc aaattgatac taatggctca
    14281 acgtgataag gaaaaagaat tgcactttaa cattaatatt gacaaggagg agggcaccac
    14341 acaaaaagtt aggtgtaaca gaaaatcatg aaactatgat tcctaattta tatattggag
    14401 gattttctct aaaaaaaaaa aaatacaaca aataaaaaac actcaatgac ctgaccattt
    14461 gatggagttt aagtcaatac cttcttgaac catttcccat aatggtgaaa gttccctcaa
    14521 gaattttact ctgtcagaaa cggccttaac gacgtaggcc ggccatttaa atgtttaaac
    14581 ggccacgctg gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
    14641 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    14701 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    14761 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    14821 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    14881 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    14941 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    15001 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    15061 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    15121 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    15181 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
    15241 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa
    15301 ctgatataat taaattgaag ctctaatttg tgagtttagt attaggatct ctacccttgg
    15361 cgaaaagtcc tctgccaaca atgatgatat ctgatccacc acttacaact tcgtcgacgg
    15421 ttctgtactg ctgacccaat atgcgtatat ataccaatct aagtctgtgc tccttccttc
    15481 gttcttcctt ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa
    15541 aaaaagaata aaaaaaaaat gatgaattga aaaggtggta tggtgcactc tcagtacaat
    15601 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
    15661 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    15721 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
    15781 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcggcgcg
    15841 ccgaggacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    15901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    15961 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    16021 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    16081 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    16141 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    16201 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    16261 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    16321 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    16381 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    16441 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    16501 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    16561 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    16621 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    16681 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    16741 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    16801 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    16861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    16921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    16981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    17041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    17101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    17161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    17221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    17281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    17341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    17401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    17461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    17521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    17581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    17641 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    17701 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    17761 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    17821 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    17881 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    17941 ccggctccta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    18001 gaccatgatt acgccaagct cggaattaac cctcactaaa gggaacaaaa gctg
//
