LOCUS       sequence-78818-e       12328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively express nuclear localized PhyB in Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Yang X, Jost AP, Weiner OD, Tang C
  TITLE     A light-inducible organelle-targeting system for dynamically 
            activating and inactivating signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 
            10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.
  PUBMED    23761071
REFERENCE   2  (bases 1 to 12328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0126"; journalName: "Mol Biol Cell"; date: 
            "2013-08"; volume: "24"; issue: "15"; pages: "2419-30"
COMMENT     Sequence Label: PhyB-mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..12328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6786..7490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6928..6946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7196..7215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7530..7550
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8875..8894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8876..8894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8901..8918)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8901..8917)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8910..8932)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9058..9513
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9145..9164)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9355..9376
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9869..9888)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9988..10010
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10048..10066)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10151..10255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10256..10324,10325..11116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10474..10493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11287..11875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11776..11795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12029..12046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12163..12184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12199..12204,12205..12222,12223..12229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12237..12253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12242..12264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12261..12277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12261..12277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12298..12316
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaaa gcaatggttt
     4021 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     4081 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     4141 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     4201 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     4261 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     4321 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     4381 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     4441 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     4501 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     4561 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     4621 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     4681 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     4741 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     4801 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     4861 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt
     4921 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     4981 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     5041 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     5101 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     5161 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     5221 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     5281 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     5341 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     5401 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     5461 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     5521 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     5581 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     5641 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     5701 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     5761 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     5821 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg
     5881 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg
     5941 aacaggggat tgatgagtta ggtgcagttg caagagagat ggttaggctc attgagactg
     6001 caactgttcc tatattcgct gtggatgccg gaggctgcat caatggatgg aacgctaaga
     6061 ttgcagagtt gacaggtctc tcagttgaag aagctatggg gaagtctctg gtttctgatt
     6121 taatatacaa agagaatgaa gcaactgtca ataagcttct ttctcgtgct ttgagagggg
     6181 acgaggaaaa gaatgtggag gttaagctga aaactttcag ccccgaacta caagggaaag
     6241 cagtttttgt ggttgtgaat gcttgttcca gcaaggacta cttgaacaac attgtcggcg
     6301 tttgttttgt tggacaagac gttactagtc agaaaatcgt aatggataag ttcatcaaca
     6361 tacaaggaga ttacaaggct attgtacata gcccaaaccc tctaatcccg ccaatttttg
     6421 ctgctgacga gaacacgtgc tgcctggaat ggaacatggc gatggaaaag cttacgggtt
     6481 ggtctcgcag tgaagtgatt gggaaaatga ttgtcgggga agtgtttggg agctgttgca
     6541 tgctaaaggg tcctgatgct ttaaccaagt tcatgattgt attgcataat gcgattggtg
     6601 gccaagatac ggataagttc cctttcccat tctttgaccg caatgggaag tttgttcagg
     6661 ctctattgac tgcaaacaag cgggttagcc tcgagggaaa ggttattggg gctttctgtt
     6721 tcttgcaaat cccgagcgaa ttcgatagtg ctggtagtgc tggtagtgct ggtggtagtt
     6781 ccctagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcgata
     7501 gcgcaggcag tgctggtaag gctagcggtc caaagaaaaa gcgcaaggtg ggtatggctt
     7561 ctatgactgg tggtcaacaa atgtaggcgg ccgccaccgc ggtggagctc taagcaaata
     7621 gctaaattat atacgaatta atattatgat taagtgttta cgtgagtgcg atatttttat
     7681 tactatctta tacagttgta tatactctat aaaatgagtt gtctattaat taacgcgatg
     7741 aatgctttct gggtttacct ctccaacaac tctagtttac ttctcaatac attcaattgt
     7801 atttgatttg tcaatacttc atcattaatc aattctatag ttttgttttt ctcgtttatt
     7861 tccaaattta atgcatcaat tttattattc aatttgtcgt tgattttggt taatgatttt
     7921 atggtttgat ctctggcatt gattgtttgt gttagttttt cattattgat aattaaatta
     7981 tttaagttag ttatcaactc ggtgttttca agtttcaagt tttcaatttc tttagagttt
     8041 attagatttg tcaaagtttc tgaattgctt gattggtcct gtagaagagt atttgttgtt
     8101 gtggataatt gattcaattt ttgagacaat tgctggaagg cgttgaaata tctagcatca
     8161 atctcatggt ttttttcccg agagtctcgt agattcaatt gttttaatat atcttgggac
     8221 cactcttgat ttgaactcat ggaaattaaa ctgggtgttg tgttgtggtg taatgattgt
     8281 accccctttg cttataattg tgtggccacc gaagtcggtg atgctgtcgc cgaagaagtt
     8341 aagaaaatcc ttgcttaaaa agattctctt tttttatgat atttgtacat aaactttata
     8401 aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg gaatatgttc atagggtaga
     8461 cgaaactata tacgcaatct acatacattt atcaagaagg agaaaaagga ggatgtaaag
     8521 gaatacaggt aagcaaattg atactaatgg ctcaacgtga taaggaaaaa gaattgcact
     8581 ttaacattaa tattgacaag gaggagggca ccacacaaaa agttaggtgt aacagaaaat
     8641 catgaaacta tgattcctaa tttatatatt ggaggatttt ctctaaaaaa aaaaaaatac
     8701 aacaaataaa aaacactcaa tgacctgacc atttgatgga gtttaagtca ataccttctt
     8761 gaaccatttc ccataatggt gaaagttccc tcaagaattt tactctgtca gaaacggcct
     8821 taacgacgta ggccggccat ttaaatgttt aaacggccac gctggcccaa ttcgccctat
     8881 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8941 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     9001 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     9061 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9121 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9181 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9241 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9301 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9361 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9421 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9481 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     9541 tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt gaagctctaa
     9601 tttgtgagtt tagtattagg atctctaccc ttggcgaaaa gtcctctgcc aacaatgatg
     9661 atatctgatc caccacttac aacttcgtcg acggttctgt actgctgacc caatatgcgt
     9721 atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc
     9781 gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa
     9841 ttgaaaaggt ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     9901 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     9961 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
    10021 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
    10081 tcatgataat aatggtttct tagacgtcgg cgcgccgagg acgtcaggtg gcacttttcg
    10141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    10201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    10261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    10321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    10381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    10441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    10501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    10561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    10621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    10681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    10741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    10801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    10861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    10921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    10981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    11041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    11101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    11161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    11221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    11281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    11341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    11401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    11461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    11521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    11581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    11641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    11701 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    11761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    11821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    11941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    12001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    12061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    12121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    12181 tcattaggca ccccaggctt tacactttat gcttccggct cctatgttgt gtggaattgt
    12241 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcggaat
    12301 taaccctcac taaagggaac aaaagctg
//
