LOCUS       sequence-78819-e       13594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively express peroxisome localized PhyB in Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Yang X, Jost AP, Weiner OD, Tang C
  TITLE     A light-inducible organelle-targeting system for dynamically 
            activating and inactivating signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 
            10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.
  PUBMED    23761071
REFERENCE   2  (bases 1 to 13594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0126"; journalName: "Mol Biol Cell"; date: 
            "2013-08"; volume: "24"; issue: "15"; pages: "2419-30"
COMMENT     Sequence Label: PhyB-mCherry-PEX3
FEATURES             Location/Qualifiers
     source          1..13594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6786..7490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6928..6946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7196..7215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(10141..10160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10142..10160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10167..10184)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10167..10183)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10176..10198)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10324..10779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10411..10430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10621..10642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11135..11154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11254..11276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11314..11332)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11417..11521
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11522..11590,11591..12382)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11740..11759)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12553..13141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13042..13061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13295..13312
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13429..13450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13465..13470,13471..13488,13489..13495)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13503..13519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13508..13530
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13527..13543
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13527..13543
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        13564..13582
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaaa gcaatggttt
     4021 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     4081 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     4141 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     4201 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     4261 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     4321 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     4381 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     4441 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     4501 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     4561 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     4621 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     4681 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     4741 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     4801 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     4861 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt
     4921 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     4981 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     5041 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     5101 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     5161 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     5221 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     5281 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     5341 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     5401 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     5461 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     5521 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     5581 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     5641 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     5701 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     5761 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     5821 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg
     5881 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg
     5941 aacaggggat tgatgagtta ggtgcagttg caagagagat ggttaggctc attgagactg
     6001 caactgttcc tatattcgct gtggatgccg gaggctgcat caatggatgg aacgctaaga
     6061 ttgcagagtt gacaggtctc tcagttgaag aagctatggg gaagtctctg gtttctgatt
     6121 taatatacaa agagaatgaa gcaactgtca ataagcttct ttctcgtgct ttgagagggg
     6181 acgaggaaaa gaatgtggag gttaagctga aaactttcag ccccgaacta caagggaaag
     6241 cagtttttgt ggttgtgaat gcttgttcca gcaaggacta cttgaacaac attgtcggcg
     6301 tttgttttgt tggacaagac gttactagtc agaaaatcgt aatggataag ttcatcaaca
     6361 tacaaggaga ttacaaggct attgtacata gcccaaaccc tctaatcccg ccaatttttg
     6421 ctgctgacga gaacacgtgc tgcctggaat ggaacatggc gatggaaaag cttacgggtt
     6481 ggtctcgcag tgaagtgatt gggaaaatga ttgtcgggga agtgtttggg agctgttgca
     6541 tgctaaaggg tcctgatgct ttaaccaagt tcatgattgt attgcataat gcgattggtg
     6601 gccaagatac ggataagttc cctttcccat tctttgaccg caatgggaag tttgttcagg
     6661 ctctattgac tgcaaacaag cgggttagcc tcgagggaaa ggttattggg gctttctgtt
     6721 tcttgcaaat cccgagcgaa ttcgatagtg ctggtagtgc tggtagtgct ggtggtagtt
     6781 ccctagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcgata
     7501 gcgcaggcag tgctggtaag gctagcatgg ccccaaatca aagatcacgt tcgcttctgc
     7561 agagacatcg aggaaaggta ctcatttcat tgactggaat agctgcttta tttaccacag
     7621 ggtcggtggt agtgtttttc gtgaagagat ggttgtataa acagcagtta cggattactg
     7681 agcaacactt catcaaagaa cagatcaaaa gaaggttcga gcagacacag gaagactcgt
     7741 tgtacacaat atacgagcta cttcctgtat ggagaatggt tttgaatgaa aacgatttga
     7801 atttggacag catcgttacc caattaaagg accaaaagaa ccagttgact agagcaaagt
     7861 ctagtgaaag cagagaaagt tcgccgttaa aaagtaaagc tgagttgtgg aacgagttag
     7921 aactaaagag tttgatcaag ctggtgacag tgacgtacac agtatcgtcg ctgattcttt
     7981 taacaagact gcaactaaat atcctgacaa gaaacgagta cctggactcc gcaataaaat
     8041 taaccatgca gcaggaaaac tgcaacaaac tacagaatag gttctataac tgggttacat
     8101 cgtggtggag cgatccggag gacaaagcgg atgatgcaat ggtaatggca gcaaaaaagt
     8161 caaagaaaga aggccaggaa gtgtatatta atgagcaagc tttcctatca ctatcatggt
     8221 ggattcttaa caaggggtgg ttgagttaca atgaaataat tacaaatcaa atcgaaattg
     8281 aatttgacgg catacaccca agagatactc taacgttaga agaatttagt tctcgtctca
     8341 cgaacatatt ccggaacacg aattcccaaa tattccagca aaataataat aacctcacgt
     8401 ctattttgct tcctaaagat tccagtggac aggagtttct tctgtcacag acgcttgacg
     8461 cggatgcttt aacaagtttt cactctaaca cactagtttt taatcaattg gtgaatgaac
     8521 tgacccaatg tatcgaaagc acagcaacat ccatagtgtt agagtctcta ataaatgaat
     8581 cattccattt cataatgaac aaggtcggca taaagacaat agccaagaaa aaacctggcc
     8641 aagaagatca gcaacagtat caaatggcag ttttcgctat gtcgatgaaa gactgttgcc
     8701 aagaaatgct tcaaacaact gccgggtctt cccatagcgg cagcgtgaac gaatacctgg
     8761 ccactctgga ttctgtgcag ccgctcgatg atctgagcgc cagcgtatac agcaactttg
     8821 gcgtctccag ctcgttttcc ttcaagcctt aagcggccgc caccgcggtg gagctctaag
     8881 caaatagcta aattatatac gaattaatat tatgattaag tgtttacgtg agtgcgatat
     8941 ttttattact atcttataca gttgtatata ctctataaaa tgagttgtct attaattaac
     9001 gcgatgaatg ctttctgggt ttacctctcc aacaactcta gtttacttct caatacattc
     9061 aattgtattt gatttgtcaa tacttcatca ttaatcaatt ctatagtttt gtttttctcg
     9121 tttatttcca aatttaatgc atcaatttta ttattcaatt tgtcgttgat tttggttaat
     9181 gattttatgg tttgatctct ggcattgatt gtttgtgtta gtttttcatt attgataatt
     9241 aaattattta agttagttat caactcggtg ttttcaagtt tcaagttttc aatttcttta
     9301 gagtttatta gatttgtcaa agtttctgaa ttgcttgatt ggtcctgtag aagagtattt
     9361 gttgttgtgg ataattgatt caatttttga gacaattgct ggaaggcgtt gaaatatcta
     9421 gcatcaatct catggttttt ttcccgagag tctcgtagat tcaattgttt taatatatct
     9481 tgggaccact cttgatttga actcatggaa attaaactgg gtgttgtgtt gtggtgtaat
     9541 gattgtaccc cctttgctta taattgtgtg gccaccgaag tcggtgatgc tgtcgccgaa
     9601 gaagttaaga aaatccttgc ttaaaaagat tctctttttt tatgatattt gtacataaac
     9661 tttataaatg aaattcataa tagaaacgac acgaaattac aaaatggaat atgttcatag
     9721 ggtagacgaa actatatacg caatctacat acatttatca agaaggagaa aaaggaggat
     9781 gtaaaggaat acaggtaagc aaattgatac taatggctca acgtgataag gaaaaagaat
     9841 tgcactttaa cattaatatt gacaaggagg agggcaccac acaaaaagtt aggtgtaaca
     9901 gaaaatcatg aaactatgat tcctaattta tatattggag gattttctct aaaaaaaaaa
     9961 aaatacaaca aataaaaaac actcaatgac ctgaccattt gatggagttt aagtcaatac
    10021 cttcttgaac catttcccat aatggtgaaa gttccctcaa gaattttact ctgtcagaaa
    10081 cggccttaac gacgtaggcc ggccatttaa atgtttaaac ggccacgctg gcccaattcg
    10141 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
    10201 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10261 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    10321 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    10381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    10441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    10501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    10561 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    10621 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    10681 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    10741 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt attttctcct
    10801 tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag
    10861 ctctaatttg tgagtttagt attaggatct ctacccttgg cgaaaagtcc tctgccaaca
    10921 atgatgatat ctgatccacc acttacaact tcgtcgacgg ttctgtactg ctgacccaat
    10981 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
    11041 attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat
    11101 gatgaattga aaaggtggta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    11161 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    11221 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    11281 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
    11341 ttaatgtcat gataataatg gtttcttaga cgtcggcgcg ccgaggacgt caggtggcac
    11401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
    11461 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
    11521 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
    11581 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
    11641 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
    11701 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
    11761 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
    11821 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
    11881 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
    11941 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
    12001 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
    12061 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
    12121 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
    12181 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
    12241 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
    12301 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
    12361 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    12421 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    12481 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12541 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    12601 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    12661 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    12721 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    12781 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    12841 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    12901 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    12961 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    13021 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    13081 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    13141 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    13201 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    13261 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    13321 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    13381 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    13441 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctccta tgttgtgtgg
    13501 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
    13561 cggaattaac cctcactaaa gggaacaaaa gctg
//
