LOCUS       sequence-78828-e       12991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively express endosome localized PhyB in Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12991)
  AUTHORS   Yang X, Jost AP, Weiner OD, Tang C
  TITLE     A light-inducible organelle-targeting system for dynamically 
            activating and inactivating signaling in budding yeast.
  JOURNAL   Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 
            10.1091/mbc.E13-03-0126. Epub 2013 Jun 12.
  PUBMED    23761071
REFERENCE   2  (bases 1 to 12991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0126"; journalName: "Mol Biol Cell"; date: 
            "2013-08"; volume: "24"; issue: "15"; pages: "2419-30"
COMMENT     Sequence Label: PhyB-mCherry-SNF7
FEATURES             Location/Qualifiers
     source          1..12991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        276..683
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        3292..3996
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6786..7490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6928..6946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7196..7215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(9538..9557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9539..9557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9564..9581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9564..9580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9573..9595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9721..10176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9808..9827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10018..10039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10532..10551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10651..10673
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10711..10729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10814..10918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10919..10987,10988..11779)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11137..11156)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11950..12538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12439..12458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12692..12709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12826..12847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12862..12867,12868..12885,12886..12892)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12900..12916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12905..12927
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12924..12940
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12924..12940
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12961..12979
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgag gagaacttct agtatatcta
       61 catacctaat attattgcct tattaaaaat ggaatcccaa caattacatc aaaatccaca
      121 ttctcttcaa aatcaattgt cctgtacttc cttgttcatg tgtgttcaaa aacgttatat
      181 ttataggata attatactct atttctcaac aagtaattgg ttgtttggcc gagcggtcta
      241 aggcgcctga ttcaagaaat atcttgaccg cagttaactg tgggaatact caggtatcgt
      301 aagatgcaag agttcgaatc tcttagcaac cattattttt ttcctcaaca taacgagaac
      361 acacaggggc gctatcgcac agaatcaaat tcgatgactg gaaatttttt gttaatttca
      421 gaggtcgcct gacgcatata cctttttcaa ctgaaaaatt gggagaaaaa ggaaaggtga
      481 gagcgccgga accggctttt catatagaat agagaagcgt tcatgactaa atgcttgcat
      541 cacaatactt gaagttgaca atattattta aggacctatt gttttttcca ataggtggtt
      601 agcaatcgtc ttactttcta acttttctta ccttttacat ttcagcaata tatatatata
      661 tatttcaagg atataccatt ctaatgtctg cccctaagtt accaatagat agcggtttaa
      721 tataaggttt caacaagaaa tattttgtga aaatcaaaag ttaatagttt cactgtcgaa
      781 aatatgaaag tttaagtttt cagttcatcc cattgtagtt atcaaatttc tgtgtttccc
      841 gtatatgtgt gctctgtatt catcgctatc ttgaaactgg ttatgatcgt tgcgcatgtg
      901 acctgtatcg ctcaaaagag cccggcaccg gtaccgggca aaagccccta gcaagctaaa
      961 atgattcaac tttcgtagta atttgttcaa ccggtcacag taaagtcaat gactaacttt
     1021 tgaatcgagg atgtatttcc aattgaaagc aatgtatata taaaccattg tgttgaagct
     1081 tgtcttacag ttttcttgta tcctcccatt gttcatatag tacttaaagt accataatcc
     1141 ttaaagaaca aacttgaaag agaatacact atacacaact aataaataaa gaataatcat
     1201 ggctgtgacc aagacaattg tagttctacc aggtgaccat gttggtcaag aaatcactga
     1261 agaggccatt aaagttttaa atgctattca ggaatgtcgt ccagacaagg tcaatttcaa
     1321 gtttgatcat catttgatcg gtggtgctgc aattgatgcc actggtgttc cattaccaga
     1381 cgaagctttg gaggcctcta agaaagctga tgccgtgctt ctgggtgctg ttggtggtcc
     1441 aaaatggggt actggcgctg tcagaccaga acaaggtctt ttgaaaatcc gtaaggagtt
     1501 gcaattgtat gctaatctaa gaccatgtaa ttttgcatct gattccttac tagatctatc
     1561 gccattgaag cctgaaattg caagaggtac agatttcgtc gttgttagag aactagtggg
     1621 tggtatttat ttcggggaga gaaaagaaga tgaaggtgat ggtgtcgcct gggatagcga
     1681 aaagtattct gtgcccgaag ttcaaagaat cactagaatg gctgctttca tggctctgca
     1741 acacaaccca cctttaccta tttggtctct agataaagcc aacgttctag catcatcaag
     1801 attatggaga aaaacagtgg aagagacgat taagaatgaa ttcccacaat tgactgttca
     1861 acatcaattg attgattccg ctgctatgat cttggttaag aacccaactc atctaaatgg
     1921 tattattatc acaaacaata tgtttggtga tattatctcc gatgaagcct ccgtgatccc
     1981 aggttcgtta ggattacttc catctgcctc cttggcatct cttccagata agaatacggc
     2041 atttggttta tatgagccat gtcatggctc tgctccagac ttaccaaagg gaaaggttaa
     2101 ccccgtagca acaatcctat ctgctgctat gatgttgaag ttgtctctgg acctgttcga
     2161 agaaggtgaa attatagaac aagcagtcaa gaaagtgttg gattctggta tcagaacagc
     2221 ggatctaaag ggtaccaact ctactactga ggtcggtgat gcagtagcga aagctgtcag
     2281 ggaactatta gcttagactt agtagatgta cagttgcata cataagtctg aacagaaaga
     2341 agtaaatatc atatctagtt cggtttagaa atcagtgact atattggttt tgaccagatc
     2401 gcaatccttg caagcattta gtaatgacta tcaaaccggt gaatcatctg ataaggctat
     2461 attagcttgt gcattcgcat gtatcgggaa acgaacggta ccgggcccta aaacaagaag
     2521 agggttgact acatcacgat gagggggatc gaagaaatga tggtaaatga aataggaaat
     2581 caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa gtgaaaagtg
     2641 ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa ttgcacaatc
     2701 atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct gggcgtgagg
     2761 ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc gctaaggggc
     2821 gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac cacgacaact
     2881 ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga gtatactaga
     2941 agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat agagcgacca
     3001 tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa acagcaatag
     3061 ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg tctgtttgag
     3121 tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa gggaacttta
     3181 cacttctcct atgcacatat attaattaaa gtccaatgct agtagagaag gggggtaaca
     3241 cccctccgcg ctcttttccg atttttttct aaaccgtgga atatttcgga tatccttttg
     3301 ttgtttccgg gtgtacaata tggacttcct cttttctggc aaccaaaccc atacatcggg
     3361 attcctataa taccttcgtt ggtctcccta acatgtaggt ggcggagggg agatatacaa
     3421 tagaacagat accagacaag acataatggg ctaaacaaga ctacaccaat tacactgcct
     3481 cattgatggt ggtacataac gaactaatac tgtagcccta gacttgatag ccatcatcat
     3541 atcgaagttt cactaccctt tttccatttg ccatctattg aagtaataat aggcgcatgc
     3601 aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc atatccgcaa
     3661 tgacaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca
     3721 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc
     3781 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt
     3841 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta
     3901 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc
     3961 acaatatttc aagctatacc aagcatacaa tcaactatct catatacaaa gcaatggttt
     4021 ccggagtcgg gggtagtggc ggtggccgtg gcggtggccg tggcggagaa gaagaaccgt
     4081 cgtcaagtca cactcctaat aaccgaagag gaggagaaca agctcaatcg tcgggaacga
     4141 aatctctcag accaagaagc aacactgaat caatgagcaa agcaattcaa cagtacaccg
     4201 tcgacgcaag actccacgcc gttttcgaac aatccggcga atcagggaaa tcattcgact
     4261 actcacaatc actcaaaacg acgacgtacg gttcctctgt acctgagcaa cagatcacag
     4321 cttatctctc tcgaatccag cgaggtggtt acattcagcc tttcggatgt atgatcgccg
     4381 tcgatgaatc cagtttccgg atcatcggtt acagtgaaaa cgccagagaa atgttaggga
     4441 ttatgcctca atctgttcct actcttgaga aacctgagat tctagctatg ggaactgatg
     4501 tgagatcttt gttcacttct tcgagctcga ttctactcga gcgtgctttc gttgctcgag
     4561 agattacctt gttaaatccg gtttggatcc attccaagaa tactggtaaa ccgttttacg
     4621 ccattcttca taggattgat gttggtgttg ttattgattt agagccagct agaactgaag
     4681 atcctgcgct ttctattgct ggtgctgttc aatcgcagaa actcgcggtt cgtgcgattt
     4741 ctcagttaca ggctcttcct ggtggagata ttaagctttt gtgtgacact gtcgtggaaa
     4801 gtgtgaggga cttgactggt tatgatcgtg ttatggttta taagtttcat gaagatgagc
     4861 atggagaagt tgtagctgag agtaaacgag atgatttaga gccttatatt ggactgcatt
     4921 atcctgctac tgatattcct caagcgtcaa ggttcttgtt taagcagaac cgtgtccgaa
     4981 tgatagtaga ttgcaatgcc acacctgttc ttgtggtcca ggacgatagg ctaactcagt
     5041 ctatgtgctt ggttggttct actcttaggg ctcctcatgg ttgtcactct cagtatatgg
     5101 ctaacatggg atctattgcg tctttagcaa tggcggttat aatcaatgga aatgaagatg
     5161 atgggagcaa tgtagctagt ggaagaagct cgatgaggct ttggggtttg gttgtttgcc
     5221 atcacacttc ttctcgctgc ataccgtttc cgctaaggta tgcttgtgag tttttgatgc
     5281 aggctttcgg tttacagtta aacatggaat tgcagttagc tttgcaaatg tcagagaaac
     5341 gcgttttgag aacgcagaca ctgttatgtg atatgcttct gcgtgactcg cctgctggaa
     5401 ttgttacaca gagtcccagt atcatggact tagtgaaatg tgacggtgca gcatttcttt
     5461 accacgggaa gtattacccg ttgggtgttg ctcctagtga agttcagata aaagatgttg
     5521 tggagtggtt gcttgcgaat catgcggatt caaccggatt aagcactgat agtttaggcg
     5581 atgcggggta tcccggtgca gctgcgttag gggatgctgt gtgcggtatg gcagttgcat
     5641 atatcacaaa aagagacttt cttttttggt ttcgatctca cactgcgaaa gaaatcaaat
     5701 ggggaggcgc taagcatcat ccggaggata aagatgatgg gcaacgaatg catcctcgtt
     5761 cgtcctttca ggcttttctt gaagttgtta agagccggag tcagccatgg gaaactgcgg
     5821 aaatggatgc gattcactcg ctccagctta ttctgagaga ctcttttaaa gaatctgagg
     5881 cggctatgaa ctctaaagtt gtggatggtg tggttcagcc atgtagggat atggcggggg
     5941 aacaggggat tgatgagtta ggtgcagttg caagagagat ggttaggctc attgagactg
     6001 caactgttcc tatattcgct gtggatgccg gaggctgcat caatggatgg aacgctaaga
     6061 ttgcagagtt gacaggtctc tcagttgaag aagctatggg gaagtctctg gtttctgatt
     6121 taatatacaa agagaatgaa gcaactgtca ataagcttct ttctcgtgct ttgagagggg
     6181 acgaggaaaa gaatgtggag gttaagctga aaactttcag ccccgaacta caagggaaag
     6241 cagtttttgt ggttgtgaat gcttgttcca gcaaggacta cttgaacaac attgtcggcg
     6301 tttgttttgt tggacaagac gttactagtc agaaaatcgt aatggataag ttcatcaaca
     6361 tacaaggaga ttacaaggct attgtacata gcccaaaccc tctaatcccg ccaatttttg
     6421 ctgctgacga gaacacgtgc tgcctggaat ggaacatggc gatggaaaag cttacgggtt
     6481 ggtctcgcag tgaagtgatt gggaaaatga ttgtcgggga agtgtttggg agctgttgca
     6541 tgctaaaggg tcctgatgct ttaaccaagt tcatgattgt attgcataat gcgattggtg
     6601 gccaagatac ggataagttc cctttcccat tctttgaccg caatgggaag tttgttcagg
     6661 ctctattgac tgcaaacaag cgggttagcc tcgagggaaa ggttattggg gctttctgtt
     6721 tcttgcaaat cccgagcgaa ttcgatagtg ctggtagtgc tggtagtgct ggtggtagtt
     6781 ccctagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     6901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     6961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggtagcgata
     7501 gcgcaggcag tgctggtaag gctagcatgt ggtcatcact ttttggttgg acatcaagta
     7561 atgccaagaa taaagagtca ccaacaaagg ccatagtgcg gttgagggag catatcaacc
     7621 ttctatccaa aaagcaatcg catttacgta ctcaaattac aaatcaagag aatgaagcta
     7681 gaatcttttt gacgaagggc aataaagtaa tggcgaagaa tgcacttaaa aagaagaaga
     7741 caatcgaaca acttttaagt aaggtggaag gcacaatgga gtctatggaa cagcagctat
     7801 tctctataga aagtgcaaac ttaaatctag agacaatgag ggctatgcag gaaggtgcta
     7861 aggcaatgaa aactattcac agtggtcttg acatagataa agtggatgaa actatggacg
     7921 agataaggga gcaagtcgaa ttaggagatg aaataagcga cgctatatcc aggcctttaa
     7981 ttactggggc aaacgaggtg gatgaagatg agctggacga ggaattggac atgctggctc
     8041 aagaaaatgc taaccaagaa acgtccaaga tcgttaataa taatgttaat gcggcgccta
     8101 tctcagagaa caaagtctca ctacctagtg ttccaagtaa taaaattaaa caaagtgaga
     8161 actctgtgaa ggacggggaa gaggaagagg atgaagaaga tgaagatgaa aaagcattaa
     8221 gagaactaca agcagaaatg gggctttgag cggccgccac cgcggtggag ctctaagcaa
     8281 atagctaaat tatatacgaa ttaatattat gattaagtgt ttacgtgagt gcgatatttt
     8341 tattactatc ttatacagtt gtatatactc tataaaatga gttgtctatt aattaacgcg
     8401 atgaatgctt tctgggttta cctctccaac aactctagtt tacttctcaa tacattcaat
     8461 tgtatttgat ttgtcaatac ttcatcatta atcaattcta tagttttgtt tttctcgttt
     8521 atttccaaat ttaatgcatc aattttatta ttcaatttgt cgttgatttt ggttaatgat
     8581 tttatggttt gatctctggc attgattgtt tgtgttagtt tttcattatt gataattaaa
     8641 ttatttaagt tagttatcaa ctcggtgttt tcaagtttca agttttcaat ttctttagag
     8701 tttattagat ttgtcaaagt ttctgaattg cttgattggt cctgtagaag agtatttgtt
     8761 gttgtggata attgattcaa tttttgagac aattgctgga aggcgttgaa atatctagca
     8821 tcaatctcat ggtttttttc ccgagagtct cgtagattca attgttttaa tatatcttgg
     8881 gaccactctt gatttgaact catggaaatt aaactgggtg ttgtgttgtg gtgtaatgat
     8941 tgtaccccct ttgcttataa ttgtgtggcc accgaagtcg gtgatgctgt cgccgaagaa
     9001 gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt
     9061 ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt
     9121 agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta
     9181 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc
     9241 actttaacat taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa
     9301 aatcatgaaa ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa
     9361 tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt
     9421 cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg
     9481 ccttaacgac gtaggccggc catttaaatg tttaaacggc cacgctggcc caattcgccc
     9541 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     9601 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     9661 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     9721 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     9781 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     9841 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     9901 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     9961 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
    10021 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
    10081 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
    10141 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
    10201 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
    10261 taatttgtga gtttagtatt aggatctcta cccttggcga aaagtcctct gccaacaatg
    10321 atgatatctg atccaccact tacaacttcg tcgacggttc tgtactgctg acccaatatg
    10381 cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt
    10441 accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat
    10501 gaattgaaaa ggtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    10561 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    10621 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    10681 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
    10741 atgtcatgat aataatggtt tcttagacgt cggcgcgccg aggacgtcag gtggcacttt
    10801 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    10861 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    10921 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    10981 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    11041 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    11101 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    11161 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    11221 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    11281 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    11341 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    11401 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    11461 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    11521 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11581 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11641 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11701 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11761 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    11821 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    12001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    12061 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    12121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    12181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    12241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    12301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    12361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12601 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12661 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12721 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    12781 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    12841 cactcattag gcaccccagg ctttacactt tatgcttccg gctcctatgt tgtgtggaat
    12901 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg
    12961 aattaaccct cactaaaggg aacaaaagct g
//
