LOCUS       sequence-78843-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS10
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..19)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3..19)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      293..881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     782..801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1008..1013,1014..1030,1031..1036)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
       61 cacaagataa aaatatatca tcatgcctcc tctagaggtc tccgctcgaa acggtgcagc
      121 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
      181 ccagcaacag cggcgggaga ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
      241 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      961 ctgattctgt ggataacgca ggaaagaaca tgaattaatt ctcatgtttg acagcttatc
     1021 atcgattagc tttaatgcgg tagtttatca
//
