LOCUS       sequence-78846-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Kanamycin and erethromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Tyurin MV, Desai SG, Lynd LR
  TITLE     Electrotransformation of Clostridium thermocellum.
  JOURNAL   Appl Environ Microbiol. 2004 Feb;70(2):883-90.
  PUBMED    14766568
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2004-02"; volume: "70(2)"; pages: 
            "883-90"
COMMENT     Sequence Label: pIKM1
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(190..981)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="AKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKMT
                     DSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYEDE
                     QSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDTP
                     FKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAF
                     CVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tcgagctcgg tacccgggga tcctctagag tcgatacaaa ttcctcgtag gcgctcggga
       61 cccctatcta gcgaactttt agaaaagata taaaacatca gagtatggac agttgcggat
      121 gtacttcaga aaagattaga tgtctaaaaa gcttgtagtt aaagcttttt agacatctaa
      181 atctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
      241 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
      301 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
      361 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
      421 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
      481 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
      541 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
      601 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
      661 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
      721 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
      781 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
      841 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
      901 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
      961 cggtgatatt ctcattttag cc
//
