LOCUS       sequence-78848-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates crRNA for use in combination with tracrRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     crRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: U6-BbsI-crRNA
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..19
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4..20
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        32..431
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
     repeat_region   434..469
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   486..521
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(543..559)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(543..559)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(596..614)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(635..651)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(635..651)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(648..670)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    659..675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(683..689,690..707,708..713))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    728..749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(866..883)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctcgaggtc gacggtatcg ataagcttga tgttcgactt gcagcctgaa atacggcacg
       61 agtaggaaaa gccgagtcaa atgccgaatg cagagtctca ttacagcaca atcaactcaa
      121 gaaaaactcg acactttttt accatttgca cttaaatcct tttttattcg ttatgtatac
      181 tttttttggt ccctaaccaa aacaaaacca aactctctta gtcgtgcctc tatatttaaa
      241 actatcaatt tattatagtc aataaatcga actgtgtttt caacaaacga acaataggac
      301 actttgattc taaaggaaat tttgaaaatc ttaagcagag ggttcttaag accatttgcc
      361 aattcttata attctcaact gctctttcct gatgttgatc atttatatag gtatgttttc
      421 ctcaatactt cgggttttag agctatgctg ttttgaatgg tcccaaaacg ggtcttcgag
      481 aagacgtttt agagctatgc tgttttgaat ggtcccaaaa ctttttttac tgcagcccgg
      541 gggatccact agttctagag cggccgccac cgcggtggag ctccagcttt tgttcccttt
      601 agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
      781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
      841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      961 ataacgcagg aaagaacatg tgagcaaa
//
