LOCUS       sequence-78859-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flexed oChIEF-citrine construct in an AAV transfer vector containing
            AAV2 ITR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Lin JY, Lin MZ, Steinbach P, Tsien RY
  TITLE     Characterization of engineered channelrhodopsin variants with 
            improved properties and kinetics.
  JOURNAL   Biophys J. 2009 Mar 4;96(5):1803-14. doi: 10.1016/j.bpj.2008.11.034.
  PUBMED    19254539
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2008.11"; journalName: "Biophys J"; date: "2009-03-4-
            4"; volume: "96"; issue: "5"; pages: "1803-14"
COMMENT     Sequence Label: AAV-flex-oChIEF-citrine
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..78)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(129..162)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttgtaatcca gaggttgatt atcgataagc ttgatatcga attcataact tcgtatagca
       61 tacattatac gaagttattc tttgcaccat tctaaagaat aacagtgata atttctgggt
      121 taaggcaaat aacttcgtat aggatacttt atacgaagtt atgctagcac catggtgagc
      181 agaagaccct ggctgctggc cctggccctg gccgtggccc tggccgccgg cagcgccggc
      241 gccagcaccg gcagcgacgc caccgtgccc gtggccaccc aggacggccc cgactacgtg
      301 ttccacagag cccacgagag aatgctgttc cagaccagct acaccctgga gaacaacggc
      361 agcgtgatct gcatccccaa caacggccag tgcttctgcc tggcctggct gaagagtaac
      421 ggcaccaacg ccgagaagct ggccgccaac atcctgcagt ggatcacctt cgccctgagc
      481 gccctgtgcc tgatgttcta cggctaccag acctggaaga gtacctgcgg ctgggaggag
      541 atctacgtgg ccaccatcga gatgatcaag ttcatcatag agtacttcca cgagttcgac
      601 gagcccgccg tgatctacag cagcaacggc aacaagaccg tgtggctgag atacgccgag
      661 tggctgctga cctgccccgt ggtcctgatc cacctgagca acctgaccgg cctggccaac
      721 gactacaaca agagaaccat gggcctgctg gtgagcgaca tcggcaccat cgtgtggggc
      781 accaccgccg ccctgagcaa gggctacgtg ag
//
