LOCUS       sequence-78869-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Lin JY, Sann SB, Zhou K, Nabavi S, Proulx CD, Malinow R, Jin Y, 
            Tsien RY
  TITLE     Optogenetic inhibition of synaptic release with chromophore-assisted
            light inactivation (CALI).
  JOURNAL   Neuron. 2013 Jul 24;79(2):241-53. doi: 10.1016/j.neuron.2013.05.022.
  PUBMED    23889931
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.05"; journalName: "Neuron"; date: 
            "2013-07-24- 24"; volume: "79"; issue: "2"; pages: "241-53"
COMMENT     Sequence Label: AAV-miniSOG-VAMP2-citrine
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(42..752,753..755,756..758))
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(84..105)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     433..452
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     692..713
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tttgtaatcc agaggttgat tatcgataag cttgaattct acttgtacag ctcgtccatg
       61 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
      121 ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg cagcagcacg
      181 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
      241 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
      301 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
      361 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
      421 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
      481 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      541 gcgaagcaca tcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc
      601 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      661 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      721 accaccccgg tgaacagctc ctcgcccttg ctcaccatat cgattccccc gccgcttccg
      781 ccgctagtgc tgaagtaaac gatgatgatg atgaggatga tggcgcagat cactcccaag
      841 atgatcatca tcttgaggtt
//
