LOCUS       sequence-78874-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS2
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(14..30)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14..30)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      314..902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     803..822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1029..1034,1035..1051,1052..1057)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
       61 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagaggt ctcccatggc
      121 ctgaccccgg accaagtggt ggctatcgcc agcaacagcg gcggcaagca agcgctcgaa
      181 acggtgcagc ggctgttgcc ggtgctgtgc caggaccgag accctcgagc cacccatgac
      241 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      301 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      361 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      421 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      481 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      541 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      601 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      661 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      721 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      781 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      841 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      901 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      961 tgttctttcc tgcgttatcc cctgattctg tggataacgc aggaaagaac atgaattaat
     1021 tctcatgttt gacagcttat catcgattag ctttaatgcg gtagtttatc acagttaaat
     1081 tgctaa
//
