LOCUS       sequence-78896-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent 
            reporter of membrane voltage.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Park J, Werley CA, Venkatachalam V, Kralj JM, Dib-Hajj SD, Waxman 
            SG, Cohen AE
  TITLE     Screening fluorescent voltage indicators with spontaneously spiking 
            HEK cells.
  JOURNAL   PLoS One. 2013 Dec 31;8(12):e85221. doi: 
            10.1371/journal.pone.0085221. eCollection 2013.
  PUBMED    24391999
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-31"; volume: "8(12):e85221. doi"; pages: " 
            10.1371/journal.pone.0085221. eCollection 2013"
COMMENT     Sequence Label: Arch(D95H)-eGFP
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..808)
                     /codon_start=1
                     /product="light-driven proton pump archaerhodopsin-3 from 
                     Halorubrum sodomense (Chow et al., 2010)"
                     /label=Arch
                     /translation="MDPIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVPGIASAAYLSMFFGIGLTEVTVGGEMLDIYYARYAHWLFTTPLLL
                     LDLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYFLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGADVSAAD"
     regulatory      805..814
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcgcc cttgctcact gctactaccg gtcggtcggc ggcactgaca tcggcaccgg
       61 cactgggttc tggtgcctcg gtgtcgccca gaatagcccg ggatctcaac aggataaagc
      121 caaagccgac cttggcagtc acgtccaaca ccataaacag cagagtttcg atgcccaggc
      181 ccaccacgcc agcgccctca gtgcctatga tccacaggat agggtaagcg gtccacagca
      241 ccaagaccag agctgtcagg gtgttaaagg tagatgccac ctcggggccc cgctcctttg
      301 cagcagatcg cagggatgta gccagaaaat agagcaccac tatcatgcaa attgtagaga
      361 acaaccacca actgtatctg gctatggccg tgtggctcaa ggctccgatg aggccagtga
      421 cgatcatcag ggcgtccaca cccaccaggg tgccgatggt cacccgatcc accttagcga
      481 gaagggccag atccagcagc agaagtgggg tggtaaacag ccagtgggcg tacctggcat
      541 aatagatatc caacatttcg cccccgacgg tcacctcagt aagcccgata ccaaagaaca
      601 tagacagata tgcggcggat gcgattccgg gcaccaggat agtcacagcg taatattccc
      661 gggcatcctt atcggtgact ccccatccgc ggaccagaaa gtagaaggtt ccaatcagca
      721 tcagcagagt gccgatgccc agccacagag tttcaggtct gccgtcaccc agcaggtcgt
      781 aaccagcctg cagagcgatg gggtccatgg tggcggatcc tctagagtcg acctgcagcc
      841 caagcttcgt ctaacaaaaa agccaaaaac ggccagaatt tagcg
//
