LOCUS       sequence-78903-e        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral expression of SpCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Wang T, Wei JJ, Sabatini DM, Lander ES
  TITLE     Genetic screens in human cells using the CRISPR-Cas9 system.
  JOURNAL   Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. 
            Epub 2013 Dec 12.
  PUBMED    24336569
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1246981"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "80-4"
COMMENT     Sequence Label: pCW-Cas9
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..37
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        65..291
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             292..472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    519..644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
       61 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
      121 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      181 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      241 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      301 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      361 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      421 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      481 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      541 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      601 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      661 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      721 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      781 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      841 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      901 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      961 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1021 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1081 gcacccacca aggcaaagag aagagtggtg cagaga
//
