LOCUS       sequence-78908-e        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-inducible lentiviral expression of SpCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Wang T, Wei JJ, Sabatini DM, Lander ES
  TITLE     Genetic screens in human cells using the CRISPR-Cas9 system.
  JOURNAL   Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. 
            Epub 2013 Dec 12.
  PUBMED    24336569
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1246981"; journalName: "Science"; date: "2014-01-3-
            3"; volume: "343"; issue: "6166"; pages: "80-4"
COMMENT     Sequence Label: pCW-Cas9
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(77..124)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 acgcccagag cagccgcgtc cctgcgcaaa cccagggctg ccttggaaaa ggcgcaaccc
       61 caaccccgga tccttacttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      121 ttttgtcgcc tcccagctga gacaggtcga tccgtgtctc gtacaggccg gtgatgctct
      181 ggtggatcag ggtggcgtcc agcacctctt tggtgctggt gtacctcttc cggtcgatgg
      241 tggtgtcaaa gtacttgaag gcggcagggg ctcccagatt ggtcagggta aacaggtgga
      301 tgatattctc ggcctgctct ctgatgggct tatcccggtg cttgttgtag gcggacagca
      361 ctttgtccag attagcgtcg gccaggatca ctctcttgga gaactcgctg atctgctcga
      421 tgatctcgtc caggtagtgc ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct
      481 cgggggagcc cttcagcttc tcatagtggc tggccaggta caggaagttc acatatttgg
      541 agggcagggc cagttcgttt cccttctgca gttcgccggc agaggccagc attctcttcc
      601 ggccgttttc cagctcgaac agggagtact taggcagctt gatgatcagg tcctttttca
      661 cttctttgta gcccttggct tccagaaagt cgatgggatt cttctcgaag ctgcttcttt
      721 ccatgatggt gatccccagc agctctttca cactcttcag tttcttggac ttgccctttt
      781 ccactttggc caccaccagc acagaatagg ccacggtggg gctgtcgaag ccgccgtact
      841 tcttagggtc ccagtccttc tttctggcga tcagcttatc gctgttcctc ttgggcagga
      901 tagactcttt gctgaagccg cctgtctgca cctcggtctt tttcacgata ttcacttggg
      961 gcatgctcag cactttccgc acggtggcaa aatcccggcc cttatcccac acgatctccc
     1021 cggtttcgcc gtttgtctcg atcagaggcc gcttccggat ctcgccgttg gccagggtaa
     1081 tctcggtctt gaaaaa
//
