LOCUS       sequence-78916-e        3871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  vertebrate expression of codon-optimized Gal80 with N-terminal NLS 
            and no stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Fujimoto E, Gaynes B, Brimley CJ, Chien CB, Bonkowsky JL
  TITLE     Gal80 intersectional regulation of cell-type specific expression in 
            vertebrates.
  JOURNAL   Dev Dyn. 2011 Oct;240(10):2324-34. doi: 10.1002/dvdy.22734. Epub 
            2011 Sep 8.
  PUBMED    21905164
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.22734"; journalName: "Dev Dyn"; date: "2011-10"; 
            volume: "240"; issue: "10"; pages: "2324-34"
COMMENT     Sequence Label: pME-nlsGal80(opt)
FEATURES             Location/Qualifiers
     source          1..3871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             669..689
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1996..2095)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2112..2131)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2113..2131)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2136..2152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2136..2152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2254..2273)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2265..3074
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2332..2351)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3221..3809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3710..3729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agatggctcc aaagaagaag cgtaaggtaa tggactacaa caaaaggagc agtgtctcaa
      721 cagtgccaaa cgccgcccct attagagtgg gattcgtggg tctgaacgca gcaaagggat
      781 gggccattaa gacccactac cctgcaatcc tgcagctgag ctctcagttc cagattactg
      841 ctctgtattc tccaaagatc gaaacatcca ttgccaccat ccagagactg aaactgtcta
      901 acgctactgc cttcccaaca ctggagtcct ttgcttcctc aagtaccatc gacatgatcg
      961 tgattgctat ccaggtcgcc tcacattacg aggtggtcat gcccctgctg gagttcagca
     1021 agaacaatcc taacctgaaa tatctgtttg tggagtgggc actggcttgt tcactggatc
     1081 aggccgagag tatctacaaa gctgccgcag aacgcggcgt ccagactatc attagcctgc
     1141 agggtcgcaa gtctccctac attctgagag ccaaagagct gatcagtcag ggatatattg
     1201 gcgacatcaa cagcattgag atcgctggta atggaggctg gtacggatat gaaagaccag
     1261 tgaagagccc caaatacatt tatgagatcg gcaacggtgt ggacctggtc acaaccactt
     1321 tcggacacac aattgacatc ctgcagtaca tgaccagctc ttatttcagc agaatcaacg
     1381 ctatggtgtt taacaatatt cctgagcagg aactgatcga cgagagagga aataggctgg
     1441 gccagagggt gcctaagact gtcccagatc acctgctgtt tcagggcaca ctgctgaacg
     1501 gtaatgtgcc agtctcctgc tcattcaagg gtggaaaacc caccaagaaa tttactaaaa
     1561 acctggtgat tgacatccac ggaacaaagg gagacctgaa actggagggc gatgccggtt
     1621 tcgcagaaat ctccaatctg gtgctgtact attcaggaac aagggcaaac gattttcccc
     1681 tggcaaatgg ccagcaggct cctctggacc caggttatga tgcaggaaag gagatcatgg
     1741 aagtgtacca cctgaggaac tataatgcta ttgtcggcaa catccaccgc ctgtaccaga
     1801 gtattagcga tttccatttc aacacaaaga aaatccccga actgccttct cagttcgtga
     1861 tgcagggatt cgactttgag ggctttccta ccctgatgga tgctctgatt ctgcatagac
     1921 tgatcgagtc cgtgtacaaa tctaacatga tgggctccac tctgaacgtc tcaaatatct
     1981 ctcactactc actgtaccca gctttcttgt acaaagttgg cattataaga aagcattgct
     2041 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     2101 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     2161 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaataata tcatcatgaa
     2221 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     2281 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     2341 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     2401 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     2461 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     2521 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     2581 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     2641 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     2701 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     2761 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     2821 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     2881 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     2941 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     3001 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3061 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3121 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     3181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3841 tggccttttg ctggcctttt gctcacatgt t
//
