LOCUS       sequence-78925-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Batth TS, Singh P, Ramakrishnan VR, Sousa MM, Chan LJ, Tran HM, 
            Luning EG, Pan EH, Vuu KM, Keasling JD, Adams PD, Petzold CJ
  TITLE     A targeted proteomics toolkit for High-throughput absolute 
            quantification of Escherichia coli proteins.
  JOURNAL   Metab Eng. 2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004.
  PUBMED    25205128
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2014 Sep 6. pii: S1096-7176(14)00114-1. doi: 
            10.1016/j.ymben.2014.08.004."
COMMENT     Sequence Label: pET29b_Qcat18
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagttt cgggatggaa
       61 aaactcaggt catcaatcag cagcttcgcg cccgggaaaa agctcaggct aatatttttg
      121 tcctgtgctg ccgaacccag tgccacgacg cgtgacagcc aatcgatagc gttacgctgt
      181 gccagcagca ccagttcttg ttccagacgg aagtacgttt ccagatattt gtcaccaacg
      241 gtatcaatgg cacctgccca cacttgacga cccgagactg caaccacttg gtagcccagc
      301 ttaaacacat acaggtcatc tgaaatacgc agcgtgaccc aggtgtcatc tttataggca
      361 tccggctgcg cgacacccag cgaaactgag cggatttcat tcaggccagc tttggacggc
      421 agttcactga acaggtttgc cagagctgca cgagcctgaa aacctgccac gcccagcagg
      481 accttagaaa tggcgctcag ggtgtcaccc gatttcgctt cttgtgacag accatcgccc
      541 gtaacggttg cacgattgac aactgccacc catgcaccac gatcgattgc tgcctgtgcc
      601 agcggcaggt ctgcattctt gttatcggag gcaccattgt tagccagact tgccgtcacg
      661 gttttaccgc ccaggtgcgg ccatgccggc agccagcttt cagccttata cagatctgcc
      721 acgactttca cggcttcacc gatcgcgttg aatttccaag acagcggcgc gatggtaata
      781 accggatcac cacgaccgct ctgagcttca ccggacagac taattgcacg c
//
