LOCUS       sequence-78943-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses constituvely active cofilin fused to EGFP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Garvalov BK, Flynn KC, Neukirchen D, Meyn L, Teusch N, Wu X, 
            Brakebusch C, Bamburg JR, Bradke F
  TITLE     Cdc42 regulates cofilin during the establishment of neuronal 
            polarity.
  JOURNAL   J Neurosci. 2007 Nov 28;27(48):13117-29.
  PUBMED    18045906
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2007-11-28"; volume: "27(48)"; pages: "13117-29"
COMMENT     Sequence Label: pEGFP-N1 human cofilin S3A
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      548..557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(599..620)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctcgagctca agcttcgaat tcaccaccat ggccgccggt gtggctgtct ctgatggtgt
       61 catcaaggtg ttcaacgaca tgaaggtgcg taagtcttca acgccagagg aggtgaagaa
      121 gcgcaagaag gcggtgctct tctgcctgag tgaggacaag aagaacatca tcctggagga
      181 gggcaaggag atcctggtgg gcgatgtggg ccagactgtc gacgatccct acgccacctt
      241 tgtcaagatg ctgccagata aggactgccg ctatgccctc tatgatgcaa cctatgagac
      301 caaggagagc aagaaggagg atctggtgtt tatcttctgg gcccccgagt ctgcgcccct
      361 taagagcaaa atgatttatg ccagctccaa ggacgccatc aagaagaagc tgacagggat
      421 caagcatgaa ttgcaagcaa actgctacga ggaggtcaag gaccgctgca ccctggcaga
      481 gaagctgggg ggcagtgcgg tcatctccct ggagggcaag cctttgttat cccgggatcc
      541 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      601 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      661 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      721 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      781 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      841 gcgcaccatc ttcttcaagg acgacggcaa ctaca
//
