LOCUS       sequence-78952-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A backbone for adenovirus creation by the AdEasy method, similar to 
            pShuttle-CMV (Addgene #16403), except it uses a mouse cofilin 
            promoter (MCP) instead of the CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Mi J, Shaw AE, Pak CW, Walsh KP, Minamide LS, Bernstein BW, Kuhn TB,
            Bamburg JR
  TITLE     A genetically encoded reporter for real-time imaging of 
            cofilin-actin rods in living neurons.
  JOURNAL   PLoS One. 2013 Dec 31;8(12):e83609. doi: 
            10.1371/journal.pone.0083609.
  PUBMED    24391794
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-31"; volume: "8(12):e83609. doi"; pages: " 
            10.1371/journal.pone.0083609"
COMMENT     Sequence Label: ShuttleMCP
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(324..335)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aatctgaata attttgtgtt actcatagcg cgtaatactg gtaccgcttc ggcctccacc
       61 tggagctcca gcagtgtagc ccacgggtgc agtcacctgg agctcccgca gtgtagccca
      121 cgggtgcagt ctgactacaa tcccctgact acaacccccg gcatcctctg cgtgctgcct
      181 cgctcctggg gaagctcctc ttttacaccc tttattcagc ttggggaggt caagggcccc
      241 ggtttccttt gcctctgagt tgtttgcgac tttgcccttt ttttctaagg ccatggtcat
      301 tgtttcaggt cagagaaatt ctcctggagt tcccacccca gtttatttta aacttttgct
      361 ttcatataga ttctcttcag tctttgtgct aaaatgatca taaacctatc aactctggaa
      421 ttaaattgaa gtattccttt tcctgtatcg taaaagaaac ataaagaagt tttttttttg
      481 ttttgttttg ttttgttttg ttttttgatt tttcaagaca gggtttctct gtgtagccct
      541 ggctgtcctg gaactcactt tgtagaccag gcttgcctcg cactcagaaa tccgcctgcc
      601 tctgcctccc gagtgctggg attaaaggtg tgcgccacca tgcccggcca taaggaaatt
      661 tttagtgcac aactcttcac ccttccccca tgaattacgt ttgatctaac aatttgccaa
      721 acgtctttag acaacgccaa agctctcaaa ctttttatgt tagcatttcc tgttttttaa
      781 aaaatcctat ttgttgaaat tttactccac gggagcattt ctattggctt gaagacaccg
      841 ttatacttgg cgattctctt ttctgtgaga gaggatccca agctgatgat tcaaaatctt
      901 tt
//
