LOCUS       sequence-78983-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 I677C A734C mutant with an N-terminal 
            3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing 
            convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 I677C A734C (NT813)
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaagtaaggc cggccgtgag tttggcgctc ctgtcataac aagacactgt ggcctaaagt
       61 ttttcacatg gtcctccacg ccagaaagcc taatactatg ttgcagggca ttgaggtaag
      121 tcagggcttg tgtagaggat ccccaattca catcgggttt tttgtaggta acataaatat
      181 agagaccaag gactatcacg taggttagca aagcacacca ccagttaatg acgaacatta
      241 ctatgcaaca aagaattgct ccaagaagtg agatccacat attgtagtat ttaaatgccg
      301 gtcgccatcc tgggcttttg gccagtgaag catggaatac tgaaaaattg atgagggcat
      361 agctggcaag gaagaagttt gagatacatg gtgcaataac attcagttcg gcaattaaga
      421 tgaatccaag tgcaattaag aatgttaaga tgtagccacg taatggctca ttatttttcc
      481 cataaccttt agcaaacatc tggaaagcgg ggtagatgtt gtctttacat agggcctgaa
      541 atatcttggg agcactcact agggatgcta atgcgctaga aagagtggca gagaatatgc
      601 ccgcggaaat tagtggtgta aatcctgaca ccatactcat tacctgaaag ttgttcatta
      661 ggccgtagct acaaggactg gattcacaag atgaaaaatc aaagtttaat ttgcaggctg
      721 cgctagtaca gtttgttagc tctgttacga tagtgtcatt aacattacca gtggcatcgc
      781 gtacgacaca agaacctaca gacacggcaa ttcctacata aaccaatgta gtaattaaaa
      841 tggctaggag tgttcctttg gg
//
