LOCUS       sequence-78987-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 with mutated Ser and Thr residues in the 
            N-terminus and mutations at P676C I730C. Contains N-terminal 
            3xFLAG-YFP tag for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutati...
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tccacagatc atcaaaccaa cattttttgt gaaatcgtga acaagatgaa gtaaggccgg
       61 ccgtgagttt ggcgctcctg tcataacaag acactgtggc ctaaagtttt tcacatggtc
      121 ctccacgcca gaaagcctaa tactatgttg cagggcattg aggtaagtca gggcttgtgt
      181 agaggatccc caattcacat cgggtttttt gtaggtaaca taaatataga gaccaaggac
      241 tatcacgtag gttagcaaag cagcccacca gttacagacg aacattacta tgcaacaaag
      301 aattgctcca agaagtgaga tccacatatt gtagtattta aatgccggtc gccatcctgg
      361 gcttttggcc agtgaagcat ggaatactga aaaattgatg agggcatagc tggcaaggaa
      421 gaagtttgag ataatacatg caataacatt cagttcggca attaagatga atccaagtgc
      481 aattaagaat gttaagatgt agccacgtaa tggctcatta tttttcccat aacctttagc
      541 aaacatctgg aaagcggggt agatgttgtc tttacatagg gcctgaaata tcttgggagc
      601 actcactagg gatgctaatg cgctagaaag agtggcagag aatatgcccg cggaaattag
      661 tggtgtaaat cctgacacca tactcattac ctgaaagttg ttcattaggc cgtagctaca
      721 aggactggat tcacaagatg aaaaatcaaa gtttaatttg caggctgcgc tagtacagtt
      781 tgttagctct gttacgatag tgtcattaac attaccagtg gcatcgcgta cgacacaaga
      841 acctacagac acggcaattc ctacataaac caatgtagta attaaaatgg ctaggagtgt
      901 tcctttgggt atggctgact gaggatctgc aagatcacct gagatattt
//
