LOCUS       sequence-78988-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 P676C I730C mutant with an N-terminal 
            3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing 
            convenient restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgtacgtgtc cacagatcat caaaccaaca ttttttgtga aatcgtgaac aagatgaagt
       61 aaggccggcc gtgagtttgg cgctcctgtc ataacaagac actgtggcct aaagtttttc
      121 acatggtcct ccacgccaga aagcctaata ctatgttgca gggcattgag gtaagtcagg
      181 gcttgtgtag aggatcccca attcacatcg ggttttttgt aggtaacata aatatagaga
      241 ccaaggacta tcacgtaggt tagcaaagca gcccaccagt tacagacgaa cattactatg
      301 caacaaagaa ttgctccaag aagtgagatc cacatattgt agtatttaaa tgccggtcgc
      361 catcctgggc ttttggccag tgaagcatgg aatactgaaa aattgatgag ggcatagctg
      421 gcaaggaaga agtttgagat aatacatgca ataacattca gttcggcaat taagatgaat
      481 ccaagtgcaa ttaagaatgt taagatgtag ccacgtaatg gctcattatt tttcccataa
      541 cctttagcaa acatctggaa agcggggtag atgttgtctt tacatagggc ctgaaatatc
      601 ttgggagcac tcactaggga tgctaatgcg ctagaaagag tggcagagaa tatgcccgcg
      661 gaaattagtg gtgtaaatcc tgacaccata ctcattacct gaaagttgtt cattaggccg
      721 tagctacaag gactggattc acaagatgaa aaatcaaagt ttaatttgca ggctgcgcta
      781 gtacagtttg ttagctctgt tacgatagtg tcattaacat taccagtggc atcgcgtacg
      841 acacaagaac ctacagacac ggcaattcct acataaacca atgtagtaat taaaatggct
      901 aggagtgttc ctttgggtat ggctgactga ggatctgcaa gatcacctga gatatttgcg
      961 ccagccagaa ttccagttgc agcaggaaaa
//
