LOCUS       sequence-79013-e       11782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and 
            mOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11782)
  TITLE     Cohen Lab SLP001 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
FEATURES             Location/Qualifiers
     source          1..11782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3096..3854
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             3861..4568
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             4662..4718
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4719..5663
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="GGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQC
                     SSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVI
                     LEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLV
                     SDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLVL
                     AMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIH
                     EHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(5733..5735,5736..6449)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5733..5735,5736..6446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5775..5796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6036..6055)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6383..6404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6474..7062
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6527..6547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6945..6956)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7065..7081)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7066..7082)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7587..7767
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7793..7810)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7799..8023
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8069..8497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8156..8175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8366..8387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8506..8526)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8511..8840
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8691..8826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8753..8772
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8888..8935
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8954..9328
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9458..9579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9495..9514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9549..9568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9628..9644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9628..9644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9641..9663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9652..9668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9676..9682,9683..9700,9701..9706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9721..9742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9859..9876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10030..10618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10110..10129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10789..11580,11581..11649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11412..11431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11650..11754)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaggat ccaccatggt aagtatcgct ctgcaggctg
     3121 gttacgacct actgggtgac ggcagacctg aaaccctgtg gctgggcatc ggcactctgc
     3181 tgatgctgat tggaaccttc tactttctgg tccgcggatg gggagtcacc gataaggatg
     3241 cccgggaata ttacgctgtg actatcctgg tgtccggaat cgcatccgcc gcatatctgt
     3301 ctatgttctt tggtatcggg cttactgagg tgtccgtcgg gggcgaaatg ttggatatct
     3361 attatgccag gtacgcccag tggctgttta ccaccccact tctgctgctg cacctggccc
     3421 ttctcgctaa ggtggatcgg gtgaccatcg gcaccctggt gggtgtggac gccctgatga
     3481 tcgtcactgg cctcatcgga gccttgagcc acacggccat agccagatac agttggtggt
     3541 tgttctctac aatttgcatg atagtggtgc tctatgttct ggctacatcc ctgcgatctg
     3601 ctgcaaagga gcggggcccc gaggtggcat ctacctttaa caccctgaca gctctggtct
     3661 tggtgctgtg gaccgcttac cctatcctgt ggatcatagg cactgagggc gctggcgtgg
     3721 tgggcctggg catcgaaact ctgctgttta tggtgttgga cgtgactgcc aaggtcggct
     3781 ttggctttat cctgttgaga tcccgggcta ttctgggcga caccgaggca ccagaaccca
     3841 gtgccggtgc cgatacgttg atggtgagca agggcgagga gtataacatg gccatcatca
     3901 aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac gagttcgaga
     3961 tcgagggcga gggcgagggc cgcccctacg agggctttca gaccgctaag ctgaaggtga
     4021 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcatttc acctacggct
     4081 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttcaagctg tccttccccg
     4141 agggcttcaa gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg accgtgaccc
     4201 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     4261 tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     4321 ggatgtaccc cgaggacggt gccctgaagg gcaagatcaa gatgaggctg aagctgaagg
     4381 acggcggcca ctacacctcc gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     4441 tgcccggcgc ctacatcgtc gacatcaagt tggacatcac ctcccacaac gaggactaca
     4501 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     4561 tgtacaagag tagaatcaca agcgaaggcg agtacatccc cctggatcaa atagacataa
     4621 atgtaggtgg attttgttat gagaatgaag tgggctccgg agccacgaac ttctctctgt
     4681 taaagcaagc aggagacgtg gaagaaaacc ccggtcccgg cggagctcct gctccagacg
     4741 ctcacagcgc cccacctgga aacgattctg ccggaggcag tgagtaccat gccccagctg
     4801 gatatcaagt gaatccaccc taccaccccg tgcatgggta tgaggaacag tgcagctcca
     4861 tctacatcta ctatggggcc ctgtgggagc aggaaacagc taggggcttc cagtggtttg
     4921 ccgtgttcct gtctgccctg tttctggctt tctacggctg gcacgcctat aaggccagcg
     4981 tgggatggga ggaagtgtac gtgtgctccg tggagctgat caaagtgatt ctggagatct
     5041 atttcgagtt caccagtcct gctatgctgt tcctgtacgg agggaacatt accccatggc
     5101 tgagatatgc cgaatggctg ctgacatgtc ccgtgatcct gattcatctg tctaacatca
     5161 ccggcctgag tgaggcatac aataagcgga caatggctct gctggtgtcc gacctgggaa
     5221 ctatttgcat gggagtgaca gccgctctgg ccactgggtg ggtgaagtgg ctgttttact
     5281 gtatcggcct ggtgtatgga acccagacat tctacaacgc tggaatcatc tacgtggagt
     5341 cttactatat catgcctgcc ggcggctgta agaaactggt gctggccatg actgccgtgt
     5401 actattctag ttggctgatg tttcccggcc tgttcatctt tgggcctgaa ggcatgcaca
     5461 ccctgagcgt ggctgggtcc actattggcc ataccatcgc cgacctgctg tccaagaata
     5521 tttggggact gctggggcac ttcctgcgga tcaaaattca cgagcatatc attatgtacg
     5581 gcgatatcag gagaccagtg agctcccagt ttctgggacg caaggtggac gtgctggcct
     5641 tcgtgacaga ggaagataaa gtggcggccg ccaagagcag gatcaccagc gagggcgagt
     5701 acatccccct ggaccagatc gacatcaacg tggtgagcaa gggcgaggag ctgttcaccg
     5761 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5821 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5881 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5941 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     6001 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     6061 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     6121 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
     6181 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     6241 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     6301 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     6361 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     6421 ctctcggcat ggacgagctg tacaagtaag aattcgatat caagcttatc gataatcaac
     6481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     6721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     6781 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     6841 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     6901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     6961 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7021 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     7081 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7141 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7201 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7261 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7321 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     7381 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     7441 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     7501 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     7561 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7741 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     7801 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7861 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     7921 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     7981 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8041 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8101 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8161 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8221 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8281 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8341 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8401 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8461 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     8521 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8581 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8641 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     8701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     8761 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8821 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     8881 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     8941 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9001 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9061 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9121 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9181 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9241 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9301 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     9361 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9421 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9481 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     9541 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9601 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     9901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     9961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    10561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    10621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    10681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    10741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    10801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    10861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    10921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    10981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11101 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11401 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    11521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    11581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    11641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    11701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    11761 tccccgaaaa gtgccacctg ac
//
