LOCUS       sequence-79021-e        3018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUK21 (Kan).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUK21-deltaBB
FEATURES             Location/Qualifiers
     source          1..3018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..369
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPLVRGLEIRYRRGGRSRTSGSIPNSPYSESYYNSLAVVLQ
                     RRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCG
                     ISHRIRQSNHSTRPVAAH"
     primer_bind     61..77
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     61..77
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(91..110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(92..110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(117..134)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(117..133)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(126..148)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(433..452)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     565..586
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(740..759)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     859..881
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(919..937)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1059..1078)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1070..1888
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSTRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1146..1165)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2031..2619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2520..2539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2773..2790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2907..2928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    2983..2999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2988..3010
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3007..3018,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3007..3018,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 tctagaacta gtggatcgat ccccaattcg ccctatagtg agtcgtatta caattcactg
      121 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      181 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      241 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
      301 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
      361 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      421 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      481 cccgtcaagc tctaaatcgg ggtgggccat cgccctgata gacggttttt cgccctttga
      541 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
      601 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
      661 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
      721 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      781 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      841 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
      901 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatggggg
      961 ggggcacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1021 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     1081 tcaacgggaa acgtcttgct cgacgaggcc gcgattaaat tccaacatgg atgctgattt
     1141 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctaccgatt
     1201 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1261 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     1321 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     1381 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     1441 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     1501 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     1561 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     1621 taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     1681 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     1741 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     1801 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     1861 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     1921 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     1981 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2101 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2161 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     2221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2341 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2641 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2701 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2761 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2821 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2881 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2941 gctttacact ttatgcttcc gcggctcgta tgttgtgtgg aattgtgagc ggataacaat
     3001 ttcacacagg aaacagct
//
