LOCUS       sequence-79025-e        3141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial cloning vector based on pUK21 (Kan).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ
  TITLE     Yeast vectors for integration at the HO locus.
  JOURNAL   Nucleic Acids Res. 2001 Jun 15;29(12):E59-9.
  PUBMED    11410682
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001-06-15"; volume: "29(12)"; pages: "E59-9"
COMMENT     Sequence Label: pUK21-NotI
FEATURES             Location/Qualifiers
     source          1..3141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..492
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPLVRGLEIRYRRGGRQLKLRTLQVDGSPGLIKARQICLNE
                     LEFIDDIRSTSGLCGRSRTSGSIPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAH
                     PPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRPVAAH
                     "
     primer_bind     184..200
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     184..200
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(214..233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(215..233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(240..257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(240..256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(249..271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(556..575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     688..709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(863..882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     982..1004
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1042..1060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1182..1201)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1193..2011
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSTRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPG
                     KTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVD
                     ASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1269..1288)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2154..2742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2643..2662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2896..2913
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3030..3051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    3106..3122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3111..3133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3130..3141,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3130..3141,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg ggttaattaa ggcgcgccag
      121 atctgtttaa acgagctcga attcatcgat gatatcagat ccactagtgg cctatgcggc
      181 cgctctagaa ctagtggatc gatccccaat tcgccctata gtgagtcgta ttacaattca
      241 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      301 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      361 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
      421 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
      481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      541 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      601 ttccccgtca agctctaaat cggggtgggc catcgccctg atagacggtt tttcgccctt
      661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      721 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
      781 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
      841 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
      901 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
      961 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     1021 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatgg
     1081 gggggggcac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     1141 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     1201 tattcaacgg gaaacgtctt gctcgacgag gccgcgatta aattccaaca tggatgctga
     1261 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg
     1321 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1381 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1441 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1501 cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     1561 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     1621 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     1681 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     1741 gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     1801 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     1861 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     1921 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     1981 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2041 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2101 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2161 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2221 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2281 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2341 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2401 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2461 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2521 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2581 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2641 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2701 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2761 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2821 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2881 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     2941 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3001 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3061 caggctttac actttatgct tccgcggctc gtatgttgtg tggaattgtg agcggataac
     3121 aatttcacac aggaaacagc t
//
