LOCUS       sequence-79046-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 V729S mutant with an N-terminal 3xFLAG-YFP tag
            in mammalian cells. cDNA is synthetic, containing convenient 
            restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Forbush Lab human NKCC1 NT clones
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 V729S (NT553)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tccacagatc atcaaaccaa cattttttgt gaaatcgtga acaagatgaa gtaaggccgg
       61 ccgtgagttt ggcgctcctg tcataacaag acactgtggc ctaaagtttt tcacatggtc
      121 ctccacgcca gaaagcctaa tactatgttg cagggcattg aggtaagtca gggcttgtgt
      181 agaggatccc caattcacat cgggtttttt gtaggtaaca taaatataga gaccaaggac
      241 tatcacgtag gttagcaaag cagcccacca gttaatgctg aacattacta tgcaacaaag
      301 aattgctcca agaagtgaga tccacatatt gtagtattta aatgccggtc gccatcctgg
      361 gctttttgcc agtgaagcat ggaatactga aaaattgatg agggcatagc tggcaaggaa
      421 gaagtttgag ataattggtg caataacatt cagttcggca attaagatga atccaagtgc
      481 aattaagaat gttaagatgt agccacgtaa tggctcatta tttttcccat aacctttagc
      541 aaacatctgg aaagcggggt agatgttgtc tttacatagg gcctgaaata tcttgggagc
      601 actcactagg gatgctaatg cgctagaaag agtggcagag aatatgcccg cggaaattag
      661 tggtgtaaat cctgacacca tactcattac ctgaaagttg ttcattaggc cgtagctaca
      721 aggactggat tcacaagatg aaaaatcaaa gtttaatttg caggctgcgc tagtacagtt
      781 tgttagctct gttacgatag tgtcattaac attaccagtg gcatcgcgta cgacacaaga
      841 acctacagac acggcaattc ctacataaac caatgtagta attaaaatgg ctaggagtgt
      901 tcctttgggt atggctgact gaggatctgc aagatcacct ga
//
