LOCUS       sequence-79048-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human NKCC1 with truncation of N-terminus, mutations at 
            P676C I730C and C-terminus replaced with that of NBCe. Contains an 
            N-terminal 3xFLAG-YFP tag for expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Monette MY, Somasekharan S, Forbush B
  TITLE     Molecular Motions Involved in Na-K-Cl Cotransporter-mediated Ion 
            Transport and Transporter Activation Revealed by Internal 
            Crosslinking between Transmembrane Domains 10 and 11/12.
  JOURNAL   J Biol Chem. 2014 Jan 22.
  PUBMED    24451383
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2014 Jan 22."
COMMENT     Sequence Label: pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-termina...
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcaggaggtg tggcgctcca ggaaggtagg ggacctctcg cggtcggagg gcttggagtc
       61 ggacaggaag ggctgctgct ccatgatgtc catagggatc ttgatggagg gcaccttctc
      121 ggagtagggg cagtcggagt cgtcgttgtc ggagtccagg gagccttcct tatcctgctt
      181 ctgcttctcg tcctccttct ctttcttgtc cttctcgggg gatccccaat tcacatcggg
      241 ttttttgtag gtaacataaa tatagagacc aaggactatc acgtaggtta gcaaagcagc
      301 ccaccagtta cagacgaaca ttactatgca acaaagaatt gctccaagaa gtgagatcca
      361 catattgtag tatttaaatg ccggtcgcca tcctgggctt ttggccagtg aagcatggaa
      421 tactgaaaaa ttgatgaggg catagctggc aaggaagaag tttgagataa tacatgcaat
      481 aacattcagt tcggcaatta agatgaatcc aagtgcaatt aagaatgtta agatgtagcc
      541 acgtaatggc tcattatttt tcccataacc tttagcaaac atctggaaag cggggtagat
      601 gttgtcttta catagggcct gaaatatctt gggagcactc actagggatg ctaatgcgct
      661 agaaagagtg gcagagaata tgcccgcgga aattagtggt gtaaatcctg acaccatact
      721 cattacctga aagttgttca ttaggccgta gctacaagga ctggattcac aagatgaaaa
      781 atcaaagttt aatttgcagg ctgcgctagt acagtttgtt agctctgtta cgatagtgtc
      841 attaacatta ccagtggcat cgcgtacgac acaagaacct acagacacgg caattcctac
      901 ataaaccaat gtagtaatta aaatggctag gagtg
//
